Immunoprecipitation (IP)
(Mouse BLMH was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate0.5 uL anti-Mouse BLMH rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse BLMH rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 53 kDaObserved band size: 53 kDa)
Rabbit anti-Mouse BLMH Polyclonal Antibody | anti-BLMH antibody
Anti-BLMH Antibody, Rabbit Polyclonal
ELISA: 1:25,000-1:50,000
IP: 0.2-1uL/mg of lysate
Immunoprecipitation (IP)
(Mouse BLMH was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate0.5 uL anti-Mouse BLMH rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse BLMH rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 53 kDaObserved band size: 53 kDa)
Immunoprecipitation (IP)
(Mouse BLMH was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate0.5 uL anti-Mouse BLMH rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse BLMH rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 53 kDaObserved band size: 53 kDa)
Western Blot (WB)
(Anti-BLMH rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:53 kDaObserved band size:53 kDa)
Western Blot (WB)
(Anti-BLMH rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:53 kDaObserved band size:53 kDa)
NCBI and Uniprot Product Information
NCBI Description
The encoded protein is a cytoplasmic cysteine peptidase involved in inactivation of bleomycin, a glycopeptide which is a component of combination chemotherapy regimens for cancer. This encoded enzyme is highly conserved, and it contains the signature active site residues of cysteine protease papain superfamily enzymes. It is postulated that this enzyme has protective effects against bleomycin-induced pulmonary fibrosis and bleomycin tumor resistance. [provided by RefSeq, Jan 2010]
Uniprot Description
BLMH: The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B- aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity. Belongs to the peptidase C1 family.
Protein type: EC 3.4.22.40; Protease
Chromosomal Location of Human Ortholog: 11|11 B5
Cellular Component: cytoplasm; nucleus
Molecular Function: aminopeptidase activity; cysteine-type endopeptidase activity; cysteine-type peptidase activity; hydrolase activity; identical protein binding; peptidase activity
Biological Process: homocysteine catabolic process; proteolysis; response to drug; response to toxin
Research Articles on BLMH
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Product Notes
The BLMH blmh (Catalog #AAA8111893) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-BLMH Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BLMH can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:1000 ELISA: 1:25,000-1:50,000 IP: 0.2-1uL/mg of lysate. Researchers should empirically determine the suitability of the BLMH blmh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BLMH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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