Western Blot (WB)
(Western Blot (WB) analysis of Arfaptin 1 (K314) polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:SP2/0 cell lysateLane3:PC12 cell lysateLane4:Rat muscle tissue lysate)
Rabbit Arfaptin 1 Polyclonal Antibody | anti-K314 antibody
Arfaptin 1 (K314) Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
Arfaptin 1; Polyclonal Antibody; Arfaptin 1 (K314) Polyclonal Antibody; Arfaptin-1; ADP-ribosylation factor-interacting protein 1; ARFIP1; Arfaptin1; anti-K314 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Arfaptin 1 (K314) polyclonal antibody detects endogenous levels of Arfaptin 1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
341
Applicable Applications for anti-K314 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
IHC: 1:50-1:200
IF: 1:50-1:200
Immunogen
Synthetic peptide, corresponding to amino acids 300-350 of Human Arfaptin 1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of Arfaptin 1 (K314) polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:SP2/0 cell lysateLane3:PC12 cell lysateLane4:Rat muscle tissue lysate)
Western Blot (WB)
(Western Blot (WB) analysis of Arfaptin 1 (K314) polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:SP2/0 cell lysateLane3:PC12 cell lysateLane4:Rat muscle tissue lysate)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of Arfaptin 1 (K314) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of Arfaptin 1 (K314) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.)
Related Product Information for anti-K314 antibody
Arfaptin 1 is an ARF binding protein that inhibits PLD activation, vesicular trafficking and secretion. In the present report, we show that arfaptin 1 interacts with 'high speed' membranes independently of ARF. However, addition of myristoylated ARF3 (myrARF3) increases the association of arfaptin 1 with the membranes, suggesting that arfaptin 1 and ARF form a complex on the Golgi. Utilizing several deletion mutants of arfaptin 1 it is shown that the association of arfaptin 1 with myrARF3 is mediated via two binding sites on arfaptin 1. These two domains are needed for arfaptin 1 inhibition of PLD activation by myrARF3 in vitro.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 40kDa
NCBI Official Full Name
arfaptin-1 isoform 2
UniProt Protein Name
Arfaptin-1
UniProt Gene Name
ARFIP1
UniProt Entry Name
ARFP1_HUMAN
Uniprot Description
ARFIP1: Putative target protein of ADP-ribosylation factor. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: G protein regulator, misc.
Chromosomal Location of Human Ortholog: 4q31.3
Cellular Component: Golgi membrane; trans-Golgi network membrane; cytosol
Molecular Function: protein domain specific binding
Biological Process: intracellular protein transport; regulation of protein secretion
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Product Notes
The K314 arfip1 (Catalog #AAA3003384) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Arfaptin 1 (K314) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Arfaptin 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:1000 IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the K314 arfip1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Arfaptin 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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