Rabbit anti-Human ACF Polyclonal Antibody | anti-ACF antibody

ACF, CT (A1CF, ACF, ASP, APOBEC1 complementation factor, APOBEC1-stimulating protein) (Azide free) (HRP)

Cat. Num. AAA6270929

• Gene Names: A1CF; ACF; ASP; ACF64; ACF65; APOBEC1CF
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ACF; Polyclonal Antibody; CT (A1CF; ASP; APOBEC1 complementation factor; APOBEC1-stimulating protein) (Azide free) (HRP); A1CF; APOBEC1-stimulating protein; anti-ACF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 594

• Applicable Applications for anti-ACF antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ACF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 397-427 amino acids from the C-terminal region of human ACF.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ACF Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the ACF antibody detected the ACF protein (arrow).)

Immunohistochemistry (IHC)

(ACF Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ACF Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ACF Antibody (C-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ACF antibody

Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events.

Product Categories/Family for anti-ACF antibody

Antibodies; Complement

References

Galloway, C.A., et al. Biochem. Biophys. Res. Commun. 391(1):659-663(2010). Blanc, V., et al. Mol. Cell. Biol. 25(16):7260-7269(2005). Deloukas, P., et al. Nature 429(6990):375-381(2004). Xie, K., et al. Proc. Natl. Acad. Sci. U.S.A. 101(21):8114-8119(2004). Blanc, V., et al. J. Biol. Chem. 278(42):41198-41204(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 20357575
• NCBI GeneID: 29974
• NCBI Accession #: NP_620310
• NCBI GenBank Nucleotide #: NP_620310.1
• UniProt Accession #: Q9NQ94
• NCBI Official Full Name: APOBEC1 complementation factor isoform 2
• NCBI Official Synonym Full Names: APOBEC1 complementation factor
• NCBI Official Symbol: A1CF
• NCBI Official Synonym Symbols: ACF; ASP; ACF64; ACF65; APOBEC1CF
• NCBI Protein Information: APOBEC1 complementation factor
• UniProt Protein Name: APOBEC1 complementation factor
• UniProt Gene Name: A1CF
• UniProt Synonym Gene Names: ACF; ASP
• UniProt Entry Name: A1CF_HUMAN

NCBI Description

Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

ACF: Essential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also protects the edited APOB mRNA from nonsense-mediated decay. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA processing; RNA-binding; Hydrolase

Chromosomal Location of Human Ortholog: 10q11.23

Cellular Component: nucleoplasm; endoplasmic reticulum; cytoplasm; apolipoprotein B mRNA editing enzyme complex

Molecular Function: protein binding; single-stranded RNA binding; RNA binding; double-stranded RNA binding; nucleotide binding

Biological Process: protein stabilization; cytidine to uridine editing; mRNA modification; gene expression; mRNA processing