Rabbit DNA pol beta Polyclonal Antibody | anti-DNA antibody

DNA pol beta (Q324) Polyclonal Antibody

Cat. Num. AAA3000457

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: DNA pol beta; Polyclonal Antibody; DNA pol beta (Q324) Polyclonal Antibody; DNA directed DNA polymerase beta; DNA polymerase beta; DNA polymerase beta subunit; DPOLB; MGC125976; Pol B; Pol beta; PolB; Polymerase (DNA directed) beta; anti-DNA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DDNA pol beta (Q324) polyclonal antibody detects endogenous levels of DNA pol beta protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 335

• Applicable Applications for anti-DNA antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 301-350 of Human DNA pol beta.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of DNA pol beta (Q324) polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:sp2/0 cell lysateLane3:Rat brain tissue lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of DNA Polymerase DNA pol beta (Q324) pAb in paraffin-embedded human breast carcinoma tissue.)

Western Blot (WB)

(Western Blot (WB) analysis ofDNA pol beta (Q324) pAb at 1:500 dilutionLane1:AML-12 whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:L02 whole cell lysate (40ug) Lane4:HepG2 whole cell lysate (40ug))

Western Blot (WB)

(Western Blot (WB) analysis of DNA pol beta (Q324) pAb at 1:500 dilutionLane1:A549 whole cell lysate (40ug) Lane2:The spleen tissue lysate of Mouse (40ug) Lane3:The testis tissue lysate of Rat (40ug) Lane4:The lung tissue lysate of Rat (40ug))

Related Product Information for anti-DNA antibody

DNA replication, recombination and repair, all of which are necessary for genomic stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair they function to excise damaged DNA fragments and correct recombinational mismatches. These exonucleases include the family of DNA polymerases. DNA pol alpha, beta, delta and epsilon are involved in DNA replication and repair. DNA pol delta and DNA pol epsilon are multisubunit enzymes, with DNA pol delta consisting of two subunits—p125, which interacts with the sliding DNA clamp protein PCNA, and p50. The nuclear-encoded DNA pol gamma is the only DNA polymerase required for the replication of the mitochondrial DNA. DNA pol zeta is ubiquitously expressed in various tissues and mediates the cellular mechanism of damage-induced mutagenesis. DNA pol theta is a DNA polymerase-helicase that binds ATP and is involved in the repair of interstrand crosslinks.

NCBI and Uniprot Product Information

• NCBI GI #: 4505931
• NCBI GeneID: 5423
• NCBI Accession #: NP_002681.1
• NCBI GenBank Nucleotide #: NP_002681.1
• UniProt Accession #: P06746
• Molecular Weight: ~ 38kDa
• NCBI Official Full Name: DNA polymerase beta
• NCBI Official Synonym Full Names: DNA polymerase beta
• NCBI Official Symbol: POLB
• NCBI Protein Information: DNA polymerase beta
• UniProt Protein Name: DNA polymerase beta
• UniProt Gene Name: POLB
• UniProt Entry Name: DPOLB_HUMAN

NCBI Description

The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011]

Uniprot Description

POLB: Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Monomer. Interacts with APEX1, HUWE1/ARF-BP1, STUB1/CHIP and USP47. Belongs to the DNA polymerase type-X family.

Protein type: EC 2.7.7.7; Transferase; Lyase; DNA replication; DNA repair, damage

Chromosomal Location of Human Ortholog: 8p11.2

Cellular Component: nucleoplasm; microtubule; spindle microtubule; cytoplasm; nucleus

Molecular Function: protein binding; enzyme binding; metal ion binding; microtubule binding; damaged DNA binding; DNA-directed DNA polymerase activity; lyase activity

Biological Process: base-excision repair, gap-filling; neuron apoptosis; response to ethanol; DNA damage response, signal transduction resulting in induction of apoptosis; base-excision repair; pyrimidine dimer repair; nucleotide-excision repair, DNA gap filling; DNA repair; DNA-dependent DNA replication; response to DNA damage stimulus