Rabbit anti-Human, Mouse ARF4 Polyclonal Antibody | anti-ARF4 antibody

ARF4 (E106) Polyclonal Antibody

Cat. Num. AAA3003370

• Gene Names: ARF4; ARF2
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ARF4; Polyclonal Antibody; ARF4 (E106) Polyclonal Antibody; ADP ribosylation factor 2; ADP ribosylation factor 4; ARF2; anti-ARF4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ARF4 (E106) polyclonal antibody detects endogenous levels of ARF4 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 180

• Applicable Applications for anti-ARF4 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 80-130 of Human ARF4.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ARF4 (E106) pAb at 1:500 dilutionLane1:HepG2 whole cell lysate (40ug) Lane2:K562 whole cell lysate (40ug) Lane3:L02 whole cell lysate (40ug) Lane4:3T3-L1 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ARF4 (E106) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.)

Related Product Information for anti-ARF4 antibody

The ADP-ribosylation factor (ARF) protein family are structurally and functionally conserved members of the Ras superfamily of regulatory GTP-binding proteins. ARFs influence vesicle trafficking and signal transduction in eukaryotic cells. ARF-dependent regulatory mechanisms include the coordination of spectrin interactions with Golgi membranes and the association of actin to the Golgi via rho family-dependent G-protein localization (Rac, CDC42) and WASP/Arp2/3 complexes. Additionally, ARFs play a central role in maintenance of organelle integrity, assembly of coat proteins, and activation of phospholipase D. The ARF proteins are categorized as class I (ARF1, ARF2, and ARF3), class II (ARF4 and ARF5) and class III (ARF6); members of each class share a common gene organization. The human ARF1 gene maps to chromosome 1q42, contains 5 exons and 4 introns, and encodes a 181 amino acid protein.

NCBI and Uniprot Product Information

• NCBI GI #: 4502205
• NCBI GeneID: 378
• NCBI Accession #: NP_001651.1
• NCBI GenBank Nucleotide #: NP_001651.1
• UniProt Accession #: P18085
• Molecular Weight: ~ 21kDa
• NCBI Official Full Name: ADP-ribosylation factor 4
• NCBI Official Synonym Full Names: ADP ribosylation factor 4
• NCBI Official Symbol: ARF4
• NCBI Official Synonym Symbols: ARF2
• NCBI Protein Information: ADP-ribosylation factor 4
• UniProt Protein Name: ADP-ribosylation factor 4
• Protein Family: Auxin response factor
• UniProt Gene Name: ARF4
• UniProt Synonym Gene Names: ARF2
• UniProt Entry Name: ARF4_HUMAN

NCBI Description

This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ARF4: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Belongs to the small GTPase superfamily. Arf family.

Protein type: G protein, monomeric; Motility/polarity/chemotaxis; G protein, monomeric, ARF; G protein

Chromosomal Location of Human Ortholog: 3p14.3

Cellular Component: Golgi apparatus; membrane; cytosol

Molecular Function: GTPase activity; protein binding; GTP binding; epidermal growth factor receptor binding

Biological Process: vesicle-mediated transport; epidermal growth factor receptor signaling pathway; phospholipase D activation; protein transport; cell migration; protein amino acid ADP-ribosylation; small GTPase mediated signal transduction; organelle organization and biogenesis; positive regulation of transcription from RNA polymerase II promoter; brain development; response to axon injury; negative regulation of apoptosis