Rabbit TFIIA-alpha Polyclonal Antibody | anti-TFIIA antibody

TFIIA-alpha (E311) Polyclonal Antibody

Cat. Num. AAA3002405

• Gene Names: GTF2A1; TF2A1; TFIIA; TFIIAL; TFIIA-42
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TFIIA-alpha; Polyclonal Antibody; TFIIA-alpha (E311) Polyclonal Antibody; Transcription initiation factor IIA subunit 1; General transcription factor IIA subunit 1; TFIIAL; Transcription initiation factor TFIIA 42 kDa subunit; TFIIA-42; Transcription initiation factor IIA alpha chain; TFIIA p35 subunit; Transcription initiation factor IIA beta chain; TFIIA p19 subunit; GTF2A1; TF2A1; anti-TFIIA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TFIIA-alpha (E311) polyclonal antibody detects endogenous levels of TFIIA-alpha protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 376

• Applicable Applications for anti-TFIIA antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 300-350 of Human TFIIA-alpha.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of TFIIA-alpha (E311) pAb at 1:500 dilutionLane1:A549 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:SP2/0 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of TFIIA-alpha (E311) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.)

Related Product Information for anti-TFIIA antibody

Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFIIH. Recognition of the TATA binding element by TBP, one of the first steps in transcription initiation, may be regulated by TFIIA. TFIIA consists of three subunits designated TFIIA-alpha, TFIIA-beta and TFIIA-gamma, and it interacts with both TBP and a TAF (TBP-associated factor). It has been demonstrated that the basic region of TBP is essential for TFIIA-dependent function of TBP.

NCBI and Uniprot Product Information

• NCBI GI #: 7706735
• NCBI GeneID: 2957
• NCBI Accession #: NP_056943.1
• NCBI GenBank Nucleotide #: NP_056943.1
• UniProt Accession #: P52655
• Molecular Weight: ~ 42kDa
• NCBI Official Full Name: transcription initiation factor IIA subunit 1 isoform 1
• NCBI Official Synonym Full Names: general transcription factor IIA subunit 1
• NCBI Official Symbol: GTF2A1
• NCBI Official Synonym Symbols: TF2A1; TFIIA; TFIIAL; TFIIA-42
• NCBI Protein Information: transcription initiation factor IIA subunit 1
• UniProt Protein Name: Transcription initiation factor IIA subunit 1
• Protein Family: TFIIA-alpha and beta-like factor
• UniProt Gene Name: GTF2A1
• UniProt Synonym Gene Names: TF2A1; TFIIA-42
• UniProt Entry Name: TF2AA_HUMAN

NCBI Description

Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.[supplied by OMIM, Jul 2010]

Uniprot Description

GTF2A1: a general transcription factor that plays an important role in transcriptional activation. A component of the transcription machinery of RNA polymerase II. Phosphorylated by Taf(II) 250 on serines that regulate TBP binding. Interacts with TBP (the TATA-binding protein). Two isoforms, 42 kDa and 37 kDa, are produced by alternative initiation.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 14q31.1

Cellular Component: nucleoplasm; cytoplasm; transcription factor TFIIA complex

Molecular Function: protein binding; DNA binding; TATA-binding protein binding; protein heterodimerization activity; transcription coactivator activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; regulation of transcription, DNA-dependent; viral reproduction; RNA elongation from RNA polymerase II promoter; gene expression