Rabbit CCL7 Polyclonal Antibody | anti-CCL7 antibody

CCL7 Polyclonal Antibody

Cat. Num. AAA3007157

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CCL7; Polyclonal Antibody; CCL7 Polyclonal Antibody; C-C motif chemokine 7; Monocyte chemoattractant protein 3; Monocyte chemotactic protein 3; MCP-3; NC28; Small-inducible cytokine A7; MCP3; SCYA6; SCYA7; anti-CCL7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CCL7 polyclonal antibody detects endogenous levels of CCL7 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 109

• Applicable Applications for anti-CCL7 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human CCL7.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CCL7 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:sp2/0 whole cell lysateLane4:PC12 whole cell lysate)

Related Product Information for anti-CCL7 antibody

Eotaxin and the monocyte chemotactic proteins, MCP-1-5, form a subfamily of the C-Cr or beta-chemokines, which are characterized by a set of conserved adjacent cysteines. MCPs are produced by a variety of cells, including T lymphocytes, subsequent to their activation with cytokines such as IL-1, TNFalpha, and IFN-gamma. In vitro studies have shown that the MCP isoforms exhibit their chemotactic effects on different subpopulations of lymphocytes. MCP-3 has been shown to have the broadest range of influence, activating monocytes, dendritic cells, lymphocytes, NK cells, eosinophils, basophils and neutrophils.

NCBI and Uniprot Product Information

• NCBI GI #: 313708
• UniProt Accession #: P80098
• Molecular Weight: ~ 11kDa
• NCBI Official Full Name: monocyte chemotactic protein-3
• UniProt Protein Name: C-C motif chemokine 7
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL7
• UniProt Synonym Gene Names: MCP3; SCYA6; SCYA7; MCP-3
• UniProt Entry Name: CCL7_HUMAN

Uniprot Description

CCL7: Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; CCR1 chemokine receptor binding; CCR2 chemokine receptor binding; chemokine activity

Biological Process: cellular calcium ion homeostasis; regulation of cell shape; monocyte chemotaxis; cell-cell signaling; eosinophil chemotaxis; response to gamma radiation; cytoskeleton organization and biogenesis; immune response; inflammatory response; chemotaxis; signal transduction; positive regulation of cell migration