Rabbit ALX3 Polyclonal Antibody | anti-ALX3 antibody

ALX3 (R203) Polyclonal Antibody

Cat. Num. AAA3001840

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ALX3; Polyclonal Antibody; ALX3 (R203) Polyclonal Antibody; Homeobox protein aristaless-like 3; Proline-rich transcription factor ALX3; anti-ALX3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ALX3 (R203) polyclonal antibody detects endogenous levels of ALX3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 343

• Applicable Applications for anti-ALX3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 171-220 of Human ALX3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ALX3 (R203) pAb at 1:1000 dilutionLane1:HepG2 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:PMVEC whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ALX3 (R203) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50,showing nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-ALX3 antibody

Aristaless-related genes are a group of paired-related homeobox genes which play a role in regulating vertebrate embryogenesis. The homeodomain transciption factor aristaless-like 3 (ALX3) is expressed in mouse embryos from 8 days of gestation, predominantly in neural crest-derived mesenchyme and in lateral plate mesoderm. Expression analysis of human and mouse tissue reveals predominant ALX3 expression in brain tissue. The Alx3 gene maps to chromosome 1p23-p13 and encodes a 343 amino acid protein. Preferential methylation of Alx3 occurs in advanced-stage neuroblastoma and may repress ALX3 expression. Treatment with the methylation inhibitor 5-aza-2-deoxycytidine restores ALX3 expression. Alx3 (-) mice lack a phenotype distinct from wild-type mice, however Alx3/Alx4 double mutants demonstrate severe craniofacial abnormalities not present in Alx4 single mutants. Specifically, Alx3/Alx4 double mutant newborn mice have cleft nasal regions in addition to malformation of other neural crest-derived skull structures.

NCBI and Uniprot Product Information

• NCBI GI #: 113204604
• NCBI Accession #: NP_006483.2
• NCBI GenBank Nucleotide #: NP_006483.2
• UniProt Accession #: O95076
• Molecular Weight: ~ 36kDa
• NCBI Official Full Name: homeobox protein aristaless-like 3
• UniProt Protein Name: Homeobox protein aristaless-like 3
• Protein Family: Homeobox protein
• UniProt Gene Name: ALX3
• UniProt Entry Name: ALX3_HUMAN

Uniprot Description

ALX3: Transcriptional regulator with a possible role in patterning of mesoderm during development. Defects in ALX3 are the cause of frontonasal dysplasia type 1 (FND1); also called frontonasal malformation (FNM) or frontorhiny. The term frontonasal dysplasia describes an array of abnormalities affecting the eyes, forehead and nose and linked to midfacial dysraphia. The clinical picture is highly variable. Major findings include true ocular hypertelorism; broadening of the nasal root; median facial cleft affecting the nose and/or upper lip and palate; unilateral or bilateral clefting of the alae nasi; lack of formation of the nasal tip; anterior cranium bifidum occultum; a V-shaped or widow's peak frontal hairline. Belongs to the paired homeobox family.

Protein type: DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: nucleus

Molecular Function: sequence-specific DNA binding

Biological Process: regulation of apoptosis; embryonic forelimb morphogenesis; transcription, DNA-dependent; regulation of transcription, DNA-dependent; embryonic hindlimb morphogenesis; pattern specification process; embryonic skeletal morphogenesis

Disease: Frontonasal Dysplasia 1