Rabbit Annexin 6 Polyclonal Antibody | anti-A5 antibody

Annexin 6 (A5) Polyclonal Antibody

Cat. Num. AAA3000316

• Gene Names: ANXA6; p68; p70; ANX6; CBP68; CPB-II
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Annexin 6; Polyclonal Antibody; Annexin 6 (A5) Polyclonal Antibody; ANXA6; ANX6; CALPHOBINDIN-II; CBP68; CHROMOBINDIN-20; CPB-II; P68; P70; anti-A5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Annexin A6(A5) polyclonal antibody detects endogenous levels of Annexin A6 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 673

• Applicable Applications for anti-A5 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to the N-terminus of Human Annexin VI.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Annexin VI (A5) polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:sp2/0 cell lysateLane3:Rat kidney tissue lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Annexin VI (A5) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasm and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Western Blot (WB)

(Western Blot (WB) analysis of Annexin VI (A5) pAb at 1:500 dilutionLane1:The Heart tissue lysate of Mouse (40ug) Lane2:The Heart tissue lysate of Rat (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:L02 whole cell lysate (40ug))

Western Blot (WB)

(Western Blot (WB) analysis of Annexin 6 (A5) pAb at 1:500 dilutionLane1:Jurkat whole cell lysate (40ug) Lane2:THP-1 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:AML-12 whole cell lysate (40ug) Lane5:C6 whole cell lysate (40ug))

Related Product Information for anti-A5 antibody

Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. Alternatively spliced transcript variants have been described.

NCBI and Uniprot Product Information

• NCBI GI #: 71773329
• NCBI GeneID: 309
• NCBI Accession #: NP_001146.2
• NCBI GenBank Nucleotide #: NP_001146.2
• UniProt Accession #: P08133
• Molecular Weight: ~ 75kDa
• NCBI Official Full Name: annexin A6 isoform 1
• NCBI Official Synonym Full Names: annexin A6
• NCBI Official Symbol: ANXA6
• NCBI Official Synonym Symbols: p68; p70; ANX6; CBP68; CPB-II
• NCBI Protein Information: annexin A6
• UniProt Protein Name: Annexin A6
• Protein Family: G-protein coupled receptor
• UniProt Gene Name: ANXA6
• UniProt Synonym Gene Names: ANX6; CPB-II
• UniProt Entry Name: ANXA6_HUMAN

NCBI Description

Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. Alternatively spliced transcript variants have been described. [provided by RefSeq, Aug 2010]

Uniprot Description

ANXA6: a calcium/phospholipid-binding protein that may associate with CD21. May regulate the release of Ca(2+) from intracellular stores. Phosphorylated in response to growth factor stimulation. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Motility/polarity/chemotaxis; Calcium-binding; Lipid-binding

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: focal adhesion; membrane; lysosomal membrane; late endosome membrane; perinuclear region of cytoplasm; melanosome

Molecular Function: protein binding; protein homodimerization activity; GTP binding; calcium-dependent phospholipid binding; cholesterol binding; calcium ion binding; lipid binding; calcium-dependent protein binding; ligand-gated ion channel activity

Biological Process: regulation of muscle contraction; calcium ion transport; protein homooligomerization