Rabbit PKA IIalpha reg Polyclonal Antibody | anti-PKA antibody

PKA IIalpha reg (P68) Polyclonal Antibody

Cat. Num. AAA3001891

• Gene Names: PRKAR2A; PKR2; PRKAR2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PKA IIalpha reg; Polyclonal Antibody; PKA IIalpha reg (P68) Polyclonal Antibody; cAMP-dependent protein kinase type II-alpha regulatory subunit; PRKAR2A; PKR2; PRKAR2; anti-PKA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PKA IIalpha reg (P68) polyclonal antibody detects endogenous levels of PKA IIalpha reg protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 404

• Applicable Applications for anti-PKA antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Human PKA IIalpha reg.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PKA IIalpha reg (P68) pAb at 1:500 dilutionLane1:PC3 whole cell lysate (10ug) Lane2:U-87MG whole cell lysate (10ug) Lane3:The Uterus tissue lysate of Rat (40ug) Lane4:AML-12 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PKA IIalpha reg (P68) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Western Blot (WB)

(Western Blot (WB) analysis of PKA IIalpha reg (P68) pAb at 1:500 dilutionLane1:The Testis tissue lysate of Rat (40ug) Lane2:The Testis tissue lysate of Mouse (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:L02 whole cell lysate (40ug) Lane5:A549 whole cell lysate (40ug) Lane6:HCC827 whole cell lysate (40ug) Lane7:HCT116 whole cell lysate (40ug))

Related Product Information for anti-PKA antibody

The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Calpha, Cbeta and Cgamma, that each represent specific gene products. Calpha and Cbeta are closely related (93% amino acid sequence similarity), where as Cgamma displays 83% and 79% similarity to Calpha and Cbeta, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.

NCBI and Uniprot Product Information

• NCBI GI #: 4758958
• NCBI GeneID: 5576
• NCBI Accession #: NP_004148.1
• NCBI GenBank Nucleotide #: NP_004148.1
• UniProt Accession #: P13861
• Molecular Weight: ~ 50kDa
• NCBI Official Full Name: cAMP-dependent protein kinase type II-alpha regulatory subunit isoform a
• NCBI Official Synonym Full Names: protein kinase cAMP-dependent type II regulatory subunit alpha
• NCBI Official Symbol: PRKAR2A
• NCBI Official Synonym Symbols: PKR2; PRKAR2
• NCBI Protein Information: cAMP-dependent protein kinase type II-alpha regulatory subunit
• UniProt Protein Name: cAMP-dependent protein kinase type II-alpha regulatory subunit
• Protein Family: Protein lysine acetyltransferase
• UniProt Gene Name: PRKAR2A
• UniProt Synonym Gene Names: PKR2; PRKAR2
• UniProt Entry Name: KAP2_HUMAN

NCBI Description

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). [provided by RefSeq, Jul 2008]

Uniprot Description

PKAR2A: a regulatory subunit of cAMP-regulated protein kinase. The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Protein type: Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 3p21.3-p21.2

Cellular Component: centrosome; focal adhesion; membrane; cytoplasm; plasma membrane; cytosol; AMP-activated protein kinase complex; cAMP-dependent protein kinase complex

Molecular Function: protein binding; cAMP-dependent protein kinase inhibitor activity; ubiquitin protein ligase binding; cAMP-dependent protein kinase regulator activity; cAMP binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; phospholipase C activation; water transport; activation of protein kinase A; energy reserve metabolic process; renal water homeostasis; innate immune response; blood coagulation; signal transduction; regulation of insulin secretion; transmembrane transport