Rabbit Actin-alpha3/gamma2 Polyclonal Antibody | anti-E2 antibody

Actin-alpha3/gamma2 (E2) Polyclonal Antibody

Cat. Num. AAA3000297

• Gene Names: ACTG2; ACT; ACTE; VSCM; ACTA3; ACTL3; ACTSG
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Actin-alpha3/gamma2; Polyclonal Antibody; Actin-alpha3/gamma2 (E2) Polyclonal Antibody; Actin; gamma-enteric smooth muscle; Alpha-actin-3; Gamma-2-actin; Smooth muscle gamma-actin; ACTG2; ACTA3; ACTL3; ACTSG; anti-E2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Actin-alpha3/gamma2 (E2) polyclonal antibody detects endogenous levels of Actin-alpha3/gamma2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 333

• Applicable Applications for anti-E2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to the N-terminal of Human Actin-alpha3/gamma2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Actin-alpha3/gamma2 (E2) pAb at 1:500 dilutionLane1:HCT116 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:AML-12 whole cell lysate (40ug) Lane5:SGC7901 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Actin-alpha3/gamma2 (E2) pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Actin-alpha3/gamma2 (E2) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50,showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Actin-alpha3/gamma2 (E2) pAb in paraffin-embedded human lung carcinoma tissue at 1:50,showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Actin-alpha3/gamma2 (E2) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50,showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-E2 antibody

All eukaryotic cells express actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of actin are present in mammalian tissues and fall into three classes. alpha actin expression is limited to various types of muscle, whereas beta and gamma are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the actin cytoskeleton. Rho controls the assembly of actin stress fibers and focal adhesion, Rac regulates actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.

NCBI and Uniprot Product Information

• NCBI GI #: 315570273
• NCBI GeneID: 72
• NCBI Accession #: NP_001186822.1
• NCBI GenBank Nucleotide #: NP_001186822.1
• UniProt Accession #: P63267
• Molecular Weight: ~ 45kDa
• NCBI Official Full Name: actin, gamma-enteric smooth muscle isoform 2
• NCBI Official Synonym Full Names: actin gamma 2, smooth muscle
• NCBI Official Symbol: ACTG2
• NCBI Official Synonym Symbols: ACT; ACTE; VSCM; ACTA3; ACTL3; ACTSG
• NCBI Protein Information: actin, gamma-enteric smooth muscle
• UniProt Protein Name: Actin, gamma-enteric smooth muscle
• Protein Family: Regulatory protein
• UniProt Gene Name: ACTG2
• UniProt Synonym Gene Names: ACTA3; ACTL3; ACTSG
• UniProt Entry Name: ACTH_HUMAN

NCBI Description

Actins are highly conserved proteins that are involved in various types of cell motility and in the maintenance of the cytoskeleton. Three types of actins, alpha, beta and gamma, have been identified in vertebrates. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. This gene encodes actin gamma 2; a smooth muscle actin found in enteric tissues. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Based on similarity to peptide cleavage of related actins, the mature protein of this gene is formed by removal of two N-terminal peptides.[provided by RefSeq, Dec 2010]

Uniprot Description

ACTG2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Belongs to the actin family.

Protein type: Cytoskeletal; Contractile; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: extracellular space; cytoskeleton; cytosol

Molecular Function: ATP binding

Biological Process: muscle contraction

Disease: Visceral Myopathy