Rabbit Hexim1 Polyclonal Antibody | anti-Q215 antibody

Hexim1 (Q215) Polyclonal Antibody

Cat. Num. AAA3001837

• Gene Names: HEXIM1; CLP1; EDG1; HIS1; MAQ1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Hexim1; Polyclonal Antibody; Hexim1 (Q215) Polyclonal Antibody; Protein HEXIM1; Cardiac lineage protein 1; Estrogen down-regulated gene 1 protein; Hexamethylene bis-acetamide-inducible protein 1; Menage a quatre protein 1; HEXIM1; CLP1; EDG1; HIS1; MAQ1; anti-Q215 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Hexim1 (Q215) polyclonal antibody detects endogenous levels of Hexim1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 359

• Applicable Applications for anti-Q215 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 191-240 of Human Hexim1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Hexim1 (Q215) pAb at 1:1000 dilutionLane1:A549 whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:AML-12 whole cell lysate (40ug) Lane5:PMVEC whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Hexim1 (Q215) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing Cytoplasm and Nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Hexim1 (Q215) pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing Cytoplasm and Nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Hexim1 (Q215) pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing Cytoplasm and Nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-Q215 antibody

Hexamethylene bis-acetamide inducible 1 (HEXIM1) and Hexamethylene bis-acetamide inducible 2 (HEXIM2) comprise a family of proteins which inhibit positive transcription elongation factor b (P-TEFb) through association with 7SK. P-TEFb is composed of a catalytic subunit, Cdk9, and either Cyclin T1 or T2 as a regulatory subunit. This complex regulates eukaryotic gene expression at the level of elongation. The C-terminal domains of HEXIM proteins interact directly with each other. Via these domains, HEXIM1 and HEXIM2 form stable homo- and hetero-oligomers, which may aid in the formation of the 7SK small nuclear ribonucleic acid particle. Despite their similar functions, HEXIM1 and HEXIM2 exhibit distinct expression patterns in various established cell lines and human tissues.

NCBI and Uniprot Product Information

• NCBI GI #: 5453682
• NCBI GeneID: 10614
• NCBI Accession #: NP_006451.1
• NCBI GenBank Nucleotide #: NP_006451.1
• UniProt Accession #: O94992
• Molecular Weight: ~ 41kDa
• NCBI Official Full Name: protein HEXIM1
• NCBI Official Synonym Full Names: HEXIM P-TEFb complex subunit 1
• NCBI Official Symbol: HEXIM1
• NCBI Official Synonym Symbols: CLP1; EDG1; HIS1; MAQ1
• NCBI Protein Information: protein HEXIM1
• UniProt Protein Name: Protein HEXIM1
• UniProt Gene Name: HEXIM1
• UniProt Synonym Gene Names: CLP1; EDG1; HIS1; MAQ1
• UniProt Entry Name: HEXI1_HUMAN

NCBI Description

Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns. [provided by RefSeq, Jul 2008]

Uniprot Description

HEXIM1: Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Homooligomer and heterooligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with the N-CoR complex through NCOR1. Interacts with ESR1 and NR3C1. May interact with NF-kappa-B through RELA. Up-regulated by HMBA (hexamethylene bisacetamide). Down-regulated by estrogen. Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues. Belongs to the HEXIM family.

Protein type: Motility/polarity/chemotaxis; Transcription factor

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; snRNA binding

Biological Process: transcription, DNA-dependent; heart development; negative regulation of cyclin-dependent protein kinase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent