Western Blot (WB)
(Western Blot (WB) analysis of CHSY2 (R671) pAb at 1:500 dilutionLane1:PC12 whole cell lysate (40ug) Lane2:AML-12 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:L02 whole cell lysate (40ug) Lane5:A2780 whole cell lysate (40ug))
Rabbit CHSY2 Polyclonal Antibody | anti-CHSY2 antibody
CHSY2 (R671) Polyclonal Antibody
Gene Names
CHPF; CSS2; CHSY2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
CHSY2; Polyclonal Antibody; CHSY2 (R671) Polyclonal Antibody; Chondroitin sulfate synthase 2; Chondroitin glucuronyltransferase 2; Chondroitin-polymerizing factor; ChPF; Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase II; N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase II; N-acetylgalactosaminyltransferase 2; CHPF; CSS2; ORF Names: UNQ651/PRO1281; anti-CHSY2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CHSY2 (R671) polyclonal antibody detects endogenous levels of CHSY2 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
613
Applicable Applications for anti-CHSY2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
IHC: 1:50-1:200
IF: 1:50-1:200
Immunogen
Synthetic peptide, corresponding to amino acids 640-690 of Human CHSY2.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of CHSY2 (R671) pAb at 1:500 dilutionLane1:PC12 whole cell lysate (40ug) Lane2:AML-12 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:L02 whole cell lysate (40ug) Lane5:A2780 whole cell lysate (40ug))
Western Blot (WB)
(Western Blot (WB) analysis of CHSY2 (R671) pAb at 1:500 dilutionLane1:PC12 whole cell lysate (40ug) Lane2:AML-12 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:L02 whole cell lysate (40ug) Lane5:A2780 whole cell lysate (40ug))
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of CHSY2 (R671) pAb in paraffin-embedded human rectum carcinoma tissue at 1:50.showing moderate cytoplasmic and membranous staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of CHSY2 (R671) pAb in paraffin-embedded human rectum carcinoma tissue at 1:50.showing moderate cytoplasmic and membranous staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)
Related Product Information for anti-CHSY2 antibody
Chondroitin sulfate synthases (CHSYs) synthesize chondroitin sulfate, a glycosaminoglycan expressed on the surface of most cells and in extracellular matrices. Glycosaminoglycan chains are covalently linked to various of core protein families and regulate many biologic processes, including extracellular matrix deposition, cell proliferation and recognition, and morphogenesis. The CHSY family includes CHSY1, CHSY2 and CHSY3. CHSY1 and CHSY3 display both glucuronyltransferase and N-acetylgalactosaminyltransferase activities, while CHSY2 is required for chondroitin polymerizing activity. CHSY2 localizes to the Golgi apparatus and is expressed ubiquitously, with highest expression observed in pancreas, ovary, brain, heart, skeletal muscle, colon, kidney, liver, stomach, small intestine and placenta.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 85kDa
NCBI Official Full Name
chondroitin sulfate synthase 2 isoform 2
NCBI Official Synonym Full Names
chondroitin polymerizing factor
NCBI Official Symbol
CHPF
NCBI Official Synonym Symbols
CSS2; CHSY2
NCBI Protein Information
chondroitin sulfate synthase 2
UniProt Protein Name
Chondroitin sulfate synthase 2
UniProt Gene Name
CHPF
UniProt Synonym Gene Names
CSS2; ChPF
UniProt Entry Name
CHSS2_HUMAN
Uniprot Description
CHPF: a type II transmembrane protein. May act as a specific activating factor for chondroitin synthase in chondroitin biosynthesis and polymerization.
Protein type: Transferase; EC 2.4.1.226; Glycan Metabolism - chondroitin sulfate biosynthesis; Membrane protein, integral; EC 2.4.1.175
Chromosomal Location of Human Ortholog: 2q35
Cellular Component: Golgi membrane; mitochondrial matrix; integral to membrane; cytosol
Molecular Function: protein binding; N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase activity; metal ion binding; glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity
Biological Process: chondroitin sulfate metabolic process; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis
Research Articles on CHSY2
Similar Products
Product Notes
The CHSY2 chpf (Catalog #AAA3003423) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHSY2 (R671) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CHSY2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:1000 IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the CHSY2 chpf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHSY2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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