Western Blot (WB)
(ALOX15 Antibody (C-term) western blot analysis in Hela(lane 1),A549(lane 2),K562(lane 3),MDA-MB435(lane 4) cell line lysates (35ug/lane).This demonstrates the ALOX15 antibody detected the ALOX15 protein (arrow).)
Rabbit anti-Human ALOX15 Polyclonal Antibody | anti-ALOX15 antibody
ALOX15, CT (Arachidonate 15-lipoxygenase, 15-LOX, Arachidonate omega-6 Lipoxygenase, LOG15)
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:50-1:100
Western Blot (WB)
(ALOX15 Antibody (C-term) western blot analysis in Hela(lane 1),A549(lane 2),K562(lane 3),MDA-MB435(lane 4) cell line lysates (35ug/lane).This demonstrates the ALOX15 antibody detected the ALOX15 protein (arrow).)
Western Blot (WB)
(ALOX15 Antibody (C-term) western blot analysis in Hela(lane 1),A549(lane 2),K562(lane 3),MDA-MB435(lane 4) cell line lysates (35ug/lane).This demonstrates the ALOX15 antibody detected the ALOX15 protein (arrow).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lymph tissue reacted with ALOX15 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lymph tissue reacted with ALOX15 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of ALOX15 Antibody (C-term) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of ALOX15 Antibody (C-term) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
NCBI and Uniprot Product Information
Uniprot Description
ALOX15: Converts arachidonic acid to 15S- hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid. Belongs to the lipoxygenase family.
Protein type: Motility/polarity/chemotaxis; Lipid Metabolism - linoleic acid; Oxidoreductase; Lipid Metabolism - arachidonic acid; EC 1.13.11.33; EC 1.13.11.31
Chromosomal Location of Human Ortholog: 17p13.3
Cellular Component: extrinsic to internal side of plasma membrane; membrane; plasma membrane; lipid particle; cytosol
Molecular Function: hepoxilin A3 synthase activity; phosphatidylinositol-4,5-bisphosphate binding; protein binding; arachidonate 12-lipoxygenase activity; arachidonate 15-lipoxygenase activity; hepoxilin-epoxide hydrolase activity; iron ion binding
Biological Process: ossification; positive regulation of heterotypic cell-cell adhesion; wound healing; phosphatidylethanolamine biosynthetic process; leukotriene metabolic process; bone mineralization; positive regulation of actin filament polymerization; lipoxygenase pathway; negative regulation of adaptive immune response; arachidonic acid metabolic process; hepoxilin biosynthetic process; inflammatory response; apoptotic cell clearance
Research Articles on ALOX15
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Product Notes
The ALOX15 alox15 (Catalog #AAA624315) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ALOX15, CT (Arachidonate 15-lipoxygenase, 15-LOX, Arachidonate omega-6 Lipoxygenase, LOG15) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ALOX15 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:100 Immunohistochemistry: 1:50-1:100. Researchers should empirically determine the suitability of the ALOX15 alox15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALOX15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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