Typical Testing Data/Standard Curve
Mouse Arachidonate 15-lipoxygenase (ALOX15/LOG15) ELISA Kit | ALOX15 elisa kit
Mouse Arachidonate 15-lipoxygenase (ALOX15/LOG15) ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay||ALOX15/LOG15 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ALOX15/LOG15 antibody and an ALOX15/LOG15-HRP conjugate. The assay sample and buffer are incubated together with ALOX15/LOG15-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALOX15/LOG15 concentration since ALOX15/LOG15 from samples and ALOX15/LOG15-HRP conjugate compete for the anti-ALOX15/LOG15 antibody binding site. Since the number of sites is limited, as more sites are occupied by ALOX15/LOG15 from the sample, fewer sites are left to bind ALOX15/LOG15-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALOX15/LOG15 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
ALOX15: Converts arachidonic acid to 15S- hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid. Belongs to the lipoxygenase family.
Protein type: EC 1.13.11.31; Lipid Metabolism - arachidonic acid; EC 1.13.11.33; Motility/polarity/chemotaxis; Oxidoreductase; Lipid Metabolism - linoleic acid
Chromosomal Location of Human Ortholog: 17p13.3
Cellular Component: extrinsic to internal side of plasma membrane; membrane; plasma membrane; lipid particle; cytosol
Molecular Function: hepoxilin A3 synthase activity; protein binding; arachidonate 12-lipoxygenase activity; phosphatidylinositol-4,5-bisphosphate binding; arachidonate 15-lipoxygenase activity; hepoxilin-epoxide hydrolase activity; iron ion binding
Biological Process: ossification; positive regulation of heterotypic cell-cell adhesion; lipoxygenase pathway; wound healing; positive regulation of actin filament polymerization; negative regulation of adaptive immune response; phosphatidylethanolamine biosynthetic process; arachidonic acid metabolic process; leukotriene metabolic process; hepoxilin biosynthetic process; inflammatory response; bone mineralization; apoptotic cell clearance
Research Articles on ALOX15
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Product Notes
The Mouse ALOX15 alox15 (Catalog #AAA7246406) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7246406 ELISA Kit recognizes Mouse ALOX15. It is sometimes possible for the material contained within the vial of "Arachidonate 15-lipoxygenase (ALOX15/LOG15), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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