Typical Testing Data/Standard Curve (for reference only)
(Mouse CD25/IL-2sR alpha PicoKine ELISA Kit standard curve )
Mouse CD25/IL-2sR alpha ELISA Kit | IL2RA elisa kit
Mouse CD25/IL-2sR alpha PicoKine ELISA Kit
Gene Names
Il2ra; CD25; Il2r; Ly-43
Reactivity
Mouse
Synonyms
CD25/IL-2sR alpha; Mouse CD25/IL-2sR alpha PicoKine ELISA Kit; Interleukin-2 receptor subunit alpha; interleukin 2 receptor; alpha; Interleukin 2 receptor alpha chain; CD 25; CD25; CD25 antigen; IDDM10; IL 2 RA; IL 2 receptor alpha subunit; IL 2R; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2 RA; IL2-RA; IL2R; IL2RA; IL2RA_HUMAN; IL2RAC; Interleukin 2 Receptor Alpha; Interleukin 2 receptor; Ly43; p55; T Cell Growth Factor Receptor; TAC; TAC Antigen; TCGFR; IL2RA elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse CD25
Sequence Length
268
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
78pg/ml-5000pg/ml
Sensitivity
< 5pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Mouse CD25/IL-2sR alpha PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse CD25/IL-2sR alpha PicoKine ELISA Kit standard curve )
Related Product Information for IL2RA elisa kit
Principle of the assay: mouse CD25 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD25 has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD25 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD25 amount of sample captured in plate.
Background: CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. CD25 has been used as a marker to identify CD4+FoxP3+ regulatory T cells in mice. By in situ hybridization, the IL2R gene was mapped to 10p15-p14. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.
Background: CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. CD25 has been used as a marker to identify CD4+FoxP3+ regulatory T cells in mice. By in situ hybridization, the IL2R gene was mapped to 10p15-p14. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
30,723 Da
NCBI Official Full Name
Interleukin-2 receptor subunit alpha
NCBI Official Synonym Full Names
interleukin 2 receptor, alpha chain
NCBI Official Symbol
Il2ra
NCBI Official Synonym Symbols
CD25; Il2r; Ly-43
NCBI Protein Information
interleukin-2 receptor subunit alpha; IL2-RA; IL-2-RA; p55 chain; IL-2R alpha chain; IL-2R subunit alpha; IL-2 receptor subunit alpha
UniProt Protein Name
Interleukin-2 receptor subunit alpha
Protein Family
UniProt Gene Name
Il2ra
UniProt Synonym Gene Names
Il2r; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA
UniProt Entry Name
IL2RA_MOUSE
Uniprot Description
IL2RA: Receptor for interleukin-2. Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.
Protein type: Membrane protein, integral
Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane
Molecular Function: protein binding; interleukin-2 receptor activity; drug binding; interleukin-2 binding
Biological Process: Notch signaling pathway; regulation of T cell homeostatic proliferation; negative regulation of immune response; negative regulation of defense response to virus; positive regulation of activated T cell proliferation; negative regulation of lymphocyte proliferation; inflammatory response to antigenic stimulus; negative regulation of T cell proliferation; positive regulation of T cell differentiation; negative regulation of inflammatory response; T cell homeostasis; positive regulation of T cell proliferation; immune response; activated T cell apoptosis
Research Articles on IL2RA
Similar Products
Product Notes
The Mouse IL2RA il2ra (Catalog #AAA176502) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA176502 ELISA Kit recognizes Mouse IL2RA. It is sometimes possible for the material contained within the vial of "CD25/IL-2sR alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
