Rabbit ADCY 5/6 Polyclonal Antibody | anti-ADCY5/6 antibody

ADCY 5/6 (F1052) Polyclonal Antibody

Cat. Num. AAA3002293

• Gene Names: ADCY5; AC5; FDFM
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ADCY 5/6; Polyclonal Antibody; ADCY 5/6 (F1052) Polyclonal Antibody; A cyclase V; A cyclase VI; Adenylate cyclase type 5; ATP pyrophosphate-lyase 5; Adenylate cyclase type V; Adenylyl cyclase 5; ADCY5; Adenylate cyclase type 6; ATP pyrophosphate-lyase 6; Adenylate cyclase type VI; Adenylyl cyclase 6; Ca(2+)-inhibitable adenylyl cyclase; ADCY6; KIAA0422; anti-ADCY5/6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ADCY 5/6 (F1052) polyclonal antibody detects endogenous levels of Adenylate cyclase type 5 and Adenylate cyclase type 6 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 911

• Applicable Applications for anti-ADCY5/6 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 1111-1160 of Human ADCY 5
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ADCY 5/6 (F1052) polyclonal antibody at 1:500 dilutionLane1:Panc1 whole cell lysate (40ug) Lane2:PC3 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:Hela whole cell lysate (40ug) Lane5:MCF-7 whole cell lysate (40ug) Lane6:The Pancreas tissue lysate of Mouse (40ug) Lane7:The Pancreas tissue lysate of Rat (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ADCY 5/6 (F1052) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasm and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ADCY 5/6 (F1052) pAb in paraffin-embedded human stomach carcinoma tissue at 1:50,showing cytoplasm and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ADCY 5/6 (F1052) pAb in paraffin-embedded human rectum carcinoma tissue at 1:50,showing cytoplasm and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Western Blot (WB)

(Western Blot (WB) analysis of ADCY 5/6 (F1052) pAb at 1:500 dillutionLane1:HEK293T whole cell lysate (50ug) Lane2:RAW264. 7 whole cell lysate (50ug) Lane3:PC12 whole cell lysate (50ug))

Related Product Information for anti-ADCY5/6 antibody

A cyclase V, also known as ADCY5, is a 1, 261 amino acid Adenylyl cyclase that localizes to cellular membranes and contains two guanylate cyclase domains. Similar to other A cyclase proteins, A cyclase V uses magnesium as a cofactor to catalyze the conversion of ATP to cAMP. A cyclase VI, also known as ADCY6 (adenylate cyclase type 6), is a 1, 168 amino acid A cyclase that localizes to the membrane and contains two guanylate cyclase domains. Using magnesium as a cofactor, A cyclase VI functions as a calcium-inhibitable A cyclase that catalyzes the conversion of ATP to 3', 5'-cyclic AMP and diphosphate and plays a role in a variety of events throughout the body. Multiple isoforms of A cyclase VI exist due to alternative splicing events.

NCBI and Uniprot Product Information

• NCBI GI #: 314122162
• NCBI GeneID: 111
• NCBI Accession #: NP_001186571.1
• NCBI GenBank Nucleotide #: NP_001186571.1
• UniProt Accession #: O95622; O43306
• Molecular Weight: ~ 130, 139kDa
• NCBI Official Full Name: adenylate cyclase type 5 isoform 2
• NCBI Official Synonym Full Names: adenylate cyclase 5
• NCBI Official Symbol: ADCY5
• NCBI Official Synonym Symbols: AC5; FDFM
• NCBI Protein Information: adenylate cyclase type 5
• UniProt Protein Name: Adenylate cyclase type 5
• UniProt Gene Name: ADCY5
• UniProt Synonym Gene Names: AC5

NCBI Description

This gene encodes a member of the membrane-bound adenylyl cyclase enzymes. Adenylyl cyclases mediate G protein-coupled receptor signaling through the synthesis of the second messenger cAMP. Activity of the encoded protein is stimulated by the Gs alpha subunit of G protein-coupled receptors and is inhibited by protein kinase A, calcium and Gi alpha subunits. Single nucleotide polymorphisms in this gene may be associated with low birth weight and type 2 diabetes. Alternatively spliced transcript variants that encode different isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

Catalyzes the formation of the signaling molecule cAMP in response to G-protein signaling (PubMed:15385642, PubMed:26206488, PubMed:24700542). Mediates signaling downstream of ADRB1 (PubMed:24700542). Regulates the increase of free cytosolic Ca2+ in response to increased blood glucose levels and contributes to the regulation of Ca2+-dependent insulin secretion (PubMed:24740569).