Testing Data
Mouse anti-Human TIMP-4 Monoclonal Antibody | anti-TIMP-4 antibody
Mouse Anti-Human TIMP-4-UNLB
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Synonyms
TIMP-4; Monoclonal Antibody; Mouse Anti-Human TIMP-4-UNLB; Mouse Anti-Human TIMP-4 - Purified; Unlabeled Antibody; Mouse Anti-Human TIMP-4; anti-TIMP-4 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2a
Clone Number
SB30c
Specificity
Human TIMP-4
Tissue inhibitors of matrix metalloproteinases (TIMPs) constitute a family of secreted multifunctional proteins which are classified based on structural similarity to each other as well as their ability to inhibit matrix metalloproteinases (MMPs). TIMPs therefore play a critical role in ECM homeostasis but have also been shown to have erythroid-potentiating and cell growth-promoting activities. TIMP-4 (~23kDa), unlike TIMP-1, TIMP-2 and TIMP-3, shows restricted localization to cardiac, neural, breast and muscle tissue. Cardiospecific TIMP-4 seems to play a significant role in regulating the normal cell phenotype since reduced levels elicit cellular transformation. It has been shown to be an inhibitor of MMP-1, MMP- 2, MMP-3, MMP-7 and MMP-9 with a preference for MMP-2 and MMP-7. 1-9
Tissue inhibitors of matrix metalloproteinases (TIMPs) constitute a family of secreted multifunctional proteins which are classified based on structural similarity to each other as well as their ability to inhibit matrix metalloproteinases (MMPs). TIMPs therefore play a critical role in ECM homeostasis but have also been shown to have erythroid-potentiating and cell growth-promoting activities. TIMP-4 (~23kDa), unlike TIMP-1, TIMP-2 and TIMP-3, shows restricted localization to cardiac, neural, breast and muscle tissue. Cardiospecific TIMP-4 seems to play a significant role in regulating the normal cell phenotype since reduced levels elicit cellular transformation. It has been shown to be an inhibitor of MMP-1, MMP- 2, MMP-3, MMP-7 and MMP-9 with a preference for MMP-2 and MMP-7. 1-9
Form/Format
Purified (UNLB) Antibody
Sequence Length
170
Applicable Applications for anti-TIMP-4 antibody
Immunohistochemistry (Frozen Sections); Western Blot
Immunogen
Recombinant TIMP-4
Characterization
To insure lot-to-lot consistency, each batch of product is tested by IHC to conform with the characteristics of a standard reference reagent.
Preparation and Storage
The purified antibody (UNLB) is supplied as 0.1 mg purified immunoglobulin in 0.2 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
Reagents are stable for the period shown on the label if stored as directed.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
Reagents are stable for the period shown on the label if stored as directed.
Testing Data
Testing Data
Testing Data #2
Testing Data #2
References
1. Greene, J., M. Wang, Y.E. Liu, L.A. Raymond, C. Rosen, and Y.E. Shi. 1996. J. Biol. Chem. 271:30375.
2. Liu, Y.E., M. Wang, J. Greene, J. Su, S. Ullrich, H. Li, S. Sheng, P. Alexander, Q.A. Sang, and Y.E. Shi. 1997. J. Biol. Chem. 272:20479.
3. Chesler, L., D.W. Golde, N. Bersch, and M.D. Johnson. 1995. Blood 86:4506.
4. Stetler-Stevenson, W.G., N. Bersch, and D.W. Golde. 1992. FEBS Lett. 296:231.
5. Corcoran, M.L., and W.G. Stetler-Stevenson. 1995. J. Biol. Chem. 270:13453.
6. Hayakawa, T., K. Yamashita, E. Ohuchi, and A. Shinagawa. 1994. J. Cell Sci. 107:2373.
7. Leco, K.J., S.S. Apte, G.T. Taniguchi, S.P. Hawkes, R. Khokha, G.A. Schultz, and D.R. Edwards. 1997. FEBS Lett. 401:213.
8. Dollery, C.M., J.R. McEwan, M. Wang, Q.A. Sang, Y.E. Liu, and E. Shi. 1999. Circ. Res. 84:498.
9. Tummalapalli, C.M., B.J. Heath, and S.C. Tyagi. 2001. J. Cell. Biochem. 80:512.
2. Liu, Y.E., M. Wang, J. Greene, J. Su, S. Ullrich, H. Li, S. Sheng, P. Alexander, Q.A. Sang, and Y.E. Shi. 1997. J. Biol. Chem. 272:20479.
3. Chesler, L., D.W. Golde, N. Bersch, and M.D. Johnson. 1995. Blood 86:4506.
4. Stetler-Stevenson, W.G., N. Bersch, and D.W. Golde. 1992. FEBS Lett. 296:231.
5. Corcoran, M.L., and W.G. Stetler-Stevenson. 1995. J. Biol. Chem. 270:13453.
6. Hayakawa, T., K. Yamashita, E. Ohuchi, and A. Shinagawa. 1994. J. Cell Sci. 107:2373.
7. Leco, K.J., S.S. Apte, G.T. Taniguchi, S.P. Hawkes, R. Khokha, G.A. Schultz, and D.R. Edwards. 1997. FEBS Lett. 401:213.
8. Dollery, C.M., J.R. McEwan, M. Wang, Q.A. Sang, Y.E. Liu, and E. Shi. 1999. Circ. Res. 84:498.
9. Tummalapalli, C.M., B.J. Heath, and S.C. Tyagi. 2001. J. Cell. Biochem. 80:512.
NCBI and Uniprot Product Information
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Product Notes
The TIMP-4 (Catalog #AAA670156) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human TIMP-4-UNLB reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TIMP-4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (Frozen Sections); Western Blot. Researchers should empirically determine the suitability of the TIMP-4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIMP-4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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