Western Blot (WB)
(Western Blot (WB) analysis of ATG14 pAb at 1:1000 dilutionLane1:H1792 whole cell lysate (20ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug))
Rabbit ATG14 Polyclonal Antibody | anti-ATG14 antibody
ATG14 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
ATG14; Polyclonal Antibody; ATG14 Polyclonal Antibody; Beclin 1-associated autophagy-related key regulator; Barkor; Autophagy-related protein 14-like protein; Atg14L; KIAA0831; anti-ATG14 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ATG14 polyclonal antibody detects endogenous levels of ATG14 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-ATG14 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Recombination protein, corresponding to amino acids 152-406 of Human ATG14.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of ATG14 pAb at 1:1000 dilutionLane1:H1792 whole cell lysate (20ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug))
Western Blot (WB)
(Western Blot (WB) analysis of ATG14 pAb at 1:1000 dilutionLane1:H1792 whole cell lysate (20ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug))
Related Product Information for anti-ATG14 antibody
Atg14 (ATG14 autophagy related 14 homolog), also known as BARKOR (beclin 1-associated autophagy-related key regulator) or ATG14L, is a 492 amino acid protein that contains 3 coiled-coil domains in its N-terminal region. Conserved in chimpanzee, canine, bovine, mouse, rat, chicken, zebrafish, fruit fly and mosquito, Atg14 exhibits highest expression in brain and lowest expression in kidney and ovary. Atg14 localizes to isolation membranes of forming autophagosomes, cytoplasm, endoplasmic reticulum and an uncharacterized punctate structure. Necessary for both basal and inducible autophagy, Atg14 plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Atg14 promotes BECN1 translocation from the trans-Golgi network to autophagosomes, and forms a complex with BECN1, PI 3-kinase p100 and p150. Atg14 does not form complexes with KIAA0226/Rubicon or UVRAG, which forms a mutually exclusive complex with BECN1 through direct competition with Atg14.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The ATG14 (Catalog #AAA3012157) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATG14 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATG14 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the ATG14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATG14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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