Western Blot (WB)
(Western Blot (WB) analysis of MAD2L1BP polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysateLane2:Raw264. 7 cell lysateLane3:H9C2 cell lysate)
Rabbit MAD2L1BP Polyclonal Antibody | anti-MAD2L1BP antibody
MAD2L1BP Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
MAD2L1BP; Polyclonal Antibody; MAD2L1BP Polyclonal Antibody; MAD2L1-binding protein; MAD2L1 binding protein; Caught by MAD2 protein; CMT2; KIAA0110; anti-MAD2L1BP antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
MAD2L1BP polyclonal antibody detects endogenous levels of MAD2L1-binding protein protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
306
Applicable Applications for anti-MAD2L1BP antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
Synthetic peptide, corresponding to amino acids 40-85 of Human MAD2L1BP.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of MAD2L1BP polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysateLane2:Raw264. 7 cell lysateLane3:H9C2 cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of MAD2L1BP polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysateLane2:Raw264. 7 cell lysateLane3:H9C2 cell lysate)
Related Product Information for anti-MAD2L1BP antibody
MAD2L1BP (MAD2L1 binding protein), also known as CMT2, is a 274 amino acid protein that localizes to the nucleoplasm during early mitosis and to the spindle from metaphase through anaphase. Functioning as a component of the spindle checkpoint (which delays the onset of anaphase until kineotochore attachment is complete), MAD2L1BP is thought to coordinate cell cycle events in late mitosis, possibly binding to MAD2, thereby silencing the spindle checkpoint and allowing mitosis to proceed. MAD2L1BP is expressed as multiple alternative spliced isforms that, upon DNA damage, may be phosphorylated by ATM or ATR. The gene encoding MAD2L1BP maps to human chromosome 6, which contains 170 million base pairs and comprises nearly 6% of the human genome.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 34kDa
NCBI Official Full Name
MAD2L1-binding protein isoform 1
UniProt Protein Name
MAD2L1-binding protein
Protein Family
UniProt Gene Name
MAD2L1BP
UniProt Synonym Gene Names
CMT2; KIAA0110
UniProt Entry Name
MD2BP_HUMAN
Uniprot Description
MAD2L1BP: May function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex. Belongs to the MAD2L1BP family.
Protein type: Cell cycle regulation
Chromosomal Location of Human Ortholog: 6p21.1
Cellular Component: intracellular membrane-bound organelle; cytoplasm; nucleolus; spindle; nucleus
Molecular Function: protein binding
Biological Process: mitotic cell cycle checkpoint; regulation of exit from mitosis
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Product Notes
The MAD2L1BP mad2l1bp (Catalog #AAA3005968) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAD2L1BP Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MAD2L1BP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the MAD2L1BP mad2l1bp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAD2L1BP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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