Rabbit anti-Human, Mouse MTHFS Polyclonal Antibody | anti-MTHFS antibody

MTHFS, CT (MTHFS, 5-formyltetrahydrofolate cyclo-ligase, 5,10-methenyl-tetrahydrofolate synthetase) (Biotin)

Cat. Num. AAA6322749

• Gene Names: MTHFS; NEDMEHM; HsT19268
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MTHFS; Polyclonal Antibody; CT (MTHFS; 5-formyltetrahydrofolate cyclo-ligase; 5; 10-methenyl-tetrahydrofolate synthetase) (Biotin); 10-methenyl-tetrahydrofolate synthetase; anti-MTHFS antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MTHFS antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MTHFS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 132-160 amino acids from the C-terminal region of human MTHFS.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MTHFS Antibody (C-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the MTHFS antibody detected the MTHFS protein (arrow).)

Related Product Information for anti-MTHFS antibody

MTHFS contributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids.

Product Categories/Family for anti-MTHFS antibody

Antibodies; Enzymes; Synthase

References

Kelemen, L.E., et al. Cancer Epidemiol. Biomarkers Prev. 19(7):1822-1830(2010). Hazra, A., et al. Cancer Causes Control 21(3):331-345(2010). Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010): Wu, D., et al. Cancer Res. 69(18):7294-7301(2009). Franke, B., et al. Birth Defects Res. Part A Clin. Mol. Teratol. 85(3):216-226(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 5453746
• NCBI GeneID: 10588
• NCBI Accession #: NP_006432.1
• NCBI GenBank Nucleotide #: NM_006441.3
• UniProt Accession #: P49914
• NCBI Official Full Name: 5-formyltetrahydrofolate cyclo-ligase isoform a
• NCBI Official Synonym Full Names: methenyltetrahydrofolate synthetase
• NCBI Official Symbol: MTHFS
• NCBI Official Synonym Symbols: NEDMEHM; HsT19268
• NCBI Protein Information: 5-formyltetrahydrofolate cyclo-ligase
• UniProt Protein Name: 5-formyltetrahydrofolate cyclo-ligase
• UniProt Gene Name: MTHFS
• UniProt Synonym Gene Names: MTHFS; Methenyl-THF synthetase
• UniProt Entry Name: MTHFS_HUMAN

NCBI Description

The protein encoded by this gene is an enzyme that catalyzes the conversion of 5-formyltetrahydrofolate to 5,10-methenyltetrahydrofolate, a precursor of reduced folates involved in 1-carbon metabolism. An increased activity of the encoded protein can result in an increased folate turnover rate and folate depletion. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]

Uniprot Description

Contributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. Catalyzes the irreversible conversion of 5-formyltetrahydrofolate (5-FTHF) to yield 5,10-methenyltetrahydrofolate.