Rabbit Actinin-alpha2/3 Polyclonal Antibody | anti-Q58 antibody

Actinin-alpha2/3 (Q58) Polyclonal Antibody

Cat. Num. AAA3001991

• Gene Names: ACTN2; MPD6; CMH23; CMD1AA; MYOCOZ
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Actinin-alpha2/3; Polyclonal Antibody; Actinin-alpha2/3 (Q58) Polyclonal Antibody; Alpha-actinin-2; Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein; ACTN2; alpha-Actinin2; Actinin alpha2; Actininalpha2; alphaActinin-2; Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; ACTN3; alpha-Actinin3; Actinin alpha3; Actininalpha3; alphaActinin-3; Actinin-alpha3; alpha-actinin-2; alpha-actinin-3; anti-Q58 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Actinin-alpha2/3 (Q58) polyclonal antibody detects endogenous levels of actinin-alpha2 and actinin-alpha3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 686

• Applicable Applications for anti-Q58 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 30-70 of Human Actinin-alpha2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Actinin-alpha2/3 (Q58) polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:Mouse kidney tissue lysateLane3:H9C2 cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Actinin-alpha2/3 (Q58) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.showing Nucleus and Cytoplasm staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Actinin-alpha2/3 (Q58) pAb in paraffin-embedded human lung carcinoma tissue at 1:50.showing Nucleus and Cytoplasm staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Actinin-alpha2/3 (Q58) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing Nucleus and Cytoplasm staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Western Blot (WB)

(Western Blot (WB) analysis of Actinin-alpha2/3 (Q58) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:CT-26 whole cell lysate (40ug))

Western Blot (WB)

(Western Blot (WB) analysis of Actinin-alpha2/3 (Q58) pAb at 1:500 dillutionLane1:HEK293T whole cell lysateLane2:Hela whole cell lysateLane3:BV2 whole cell lysateLane4:C6 whole cell lysate)

Related Product Information for anti-Q58 antibody

The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and alpha-actinins. There are four tissue-specific alpha-actinins, namely alpha-actinin-1, alpha-actinin-2, alpha-actinin-3 and alpha-actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. Each alpha-actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellular structures. Defects in the gene encoding alpha-actinin-4 are the cause of focal segmental glomerulosclerosis 1 (FSGS1), a common renal lesion characterized by decreasing kidney function and, ultimately, renal failure.

NCBI and Uniprot Product Information

• NCBI GI #: 507588250
• NCBI GeneID: 88
• NCBI Accession #: NP_001265273.1
• NCBI GenBank Nucleotide #: NP_001265273.1
• UniProt Accession #: P35609; Q08043
• Molecular Weight: ~ 103kDa
• NCBI Official Full Name: alpha-actinin-2 isoform 3
• NCBI Official Synonym Full Names: actinin alpha 2
• NCBI Official Symbol: ACTN2
• NCBI Official Synonym Symbols: MPD6; CMH23; CMD1AA; MYOCOZ
• NCBI Protein Information: alpha-actinin-2
• UniProt Protein Name: Alpha-actinin-2
• UniProt Gene Name: ACTN2
• UniProt Entry Name: ACTN2_HUMAN

NCBI Description

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]

Uniprot Description

ACTN2: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Belongs to the alpha-actinin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 1q42-q43

Cellular Component: cortical actin cytoskeleton; cytoskeleton; focal adhesion; dendritic spine; extracellular region; actin filament; pseudopodium; Z disc; cytosol; filopodium

Molecular Function: integrin binding; protein dimerization activity; actin filament binding; identical protein binding; protein binding; LIM domain binding; structural constituent of muscle; cytoskeletal protein binding; titin binding; calcium ion binding; FATZ binding; thyroid hormone receptor coactivator activity

Biological Process: focal adhesion formation; platelet activation; negative regulation of potassium ion transport; positive regulation of potassium ion transport; muscle filament sliding; protein homotetramerization; regulation of apoptosis; synaptic transmission; regulation of membrane potential; platelet degranulation; microspike biogenesis; blood coagulation; cell adhesion

Disease: Cardiomyopathy, Dilated, 1aa