Rabbit anti-Human CD164 Polyclonal Antibody | anti-CD164 antibody

Anti-CD164 Antibody, Rabbit Polyclonal

Cat. Num. AAA8106336

• Gene Names: CD164; DFNA66; MGC-24; MUC-24; MGC-24v; endolyn
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: CD164; Polyclonal Antibody; Anti-CD164 Antibody; Rabbit Polyclonal; CD164/Endolyn Antibody; Rabbit PAb; Antigen Affinity Purified; CD164 molecule; sialomucin; Anti-endolyn Antibody; Anti-MGC-24 Antibody; Anti-MUC-24 Antibody; anti-CD164 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CD164/Endolyn
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CD164 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IHC-P: 1:5000-1:20,000; IP: 0.2-1uL/mg of lysate
• Immunogen: Recombinant Human CD164/Endolyn protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human CD164/Endolyn (rh CD164/Endolyn). CD164/Endolyn specific IgG was purified by Human CD164/Endolyn affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human CD164 in human prostatic carcinoma (from 2 donors) with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human CD164 in human pancreatic carcinoma with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

Western Blot (WB)

(Anti-CD164 rabbit polyclonal antibody at 1:500 dilution.Lane A: K562 Membrane LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:20 kDaObserved band size:85 kDa(We are unsure as to the identity of these extra bands.))

Immunoprecipitation (IP)

(CD164 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell Lysate0.5 uL anti-CD164 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CD164 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 85 kDaObserved band size: 85 kDa)

Related Product Information for anti-CD164 antibody

Sialomucin core protein 24 also known as endolyn or CD164 (cluster of differentiation 164) is a novel 80-to 90-kD mucin-like molecule expressed by human CD34+ hematopoietic progenitor cells. Isoform 1 and isoform 3 of CD164 are expressed in hematopoietic and non-hematopoietic tissues. Isoform 1 is expressed by prostate cancer tumors and prostate cancer cell lines. The expression is greater in bone metastases than in primary tumors. Expression in osseous metastasis is greater than that in soft tissue metastasis. Isoform 2 of CD164 is expressed in the small intestine, colon, lung, thyroid and colorectal and pancreatic adenocarcinoma. Isoform 4 is expressed by both hematopoietic progenitor cells and bone marrow stromal cells. CD164 belongs to the CD164 family. The cluster of differentiation (cluster of designation) (often abbreviated as CD) is a protocol used for the identification and investigation of cell surface molecules present on white blood cells initially but found in almost any kind of cell of the body, providing targets for immunophenotyping of cells. CD164 may play an important role in prostate cancer metastasis and the infiltration of bone marrow by cancer cells. CD164 promotes myogenesis by enhancing CXCR4-dependent cell motility. This protein positively regulates myoblast migration and promotes myoblast fusion into myotubes. CD164 may play a key role in hematopoiesis by facilitating the adhesion of CD34+ cells to bone marrow stroma and by negatively regulating CD34+hematopoietic progenitor cell growth.

References

McGuckin CP, et al. (2003) Colocalization analysis of sialomucins CD34 and CD164. Stem Cells. 21(2): 162-70.Doyonnas R, et al. (2000) CD164 monoclonal antibodies that block hemopoietic progenitor cell adhesion and proliferation interact with the first mucin domain of the CD164 receptor. J Immunol. 165(2): 840-51.Zannettino AC, et al. (1998) The sialomucin CD164 (MGC-24v) is an adhesive glycoprotein expressed by human hematopoietic progenitors and bone marrow stromal cells that serves as a potent negative regulator of hematopoiesis. Blood. 92(8): 2613-28.

NCBI and Uniprot Product Information

• NCBI GI #: 257051036
• NCBI GeneID: 8763
• UniProt Accession #: Q04900; O95413; Q5JSU6; Q9BPV0; Q9NR26; B4DQ85; E1P5E7; E1P5E8; E1P5E9
• Molecular Weight: 16,736 Da
• NCBI Official Full Name: Sialomucin core protein 24
• NCBI Official Synonym Full Names: CD164 molecule
• NCBI Official Symbol: CD164
• NCBI Official Synonym Symbols: DFNA66; MGC-24; MUC-24; MGC-24v; endolyn
• NCBI Protein Information: sialomucin core protein 24
• UniProt Protein Name: Sialomucin core protein 24
• Protein Family: CD164 sialomucin-like 2 protein
• UniProt Gene Name: CD164
• UniProt Synonym Gene Names: MUC-24; MGC-24; MGC-24v

NCBI Description

This gene encodes a transmembrane sialomucin and cell adhesion molecule that regulates the proliferation, adhesion and migration of hematopoietic progenitor cells. The encoded protein also interacts with the C-X-C chemokine receptor type 4 and may regulate muscle development. Elevated expression of this gene has been observed in human patients with Sezary syndrome, a type of blood cancer, and a mutation in this gene may be associated with impaired hearing. [provided by RefSeq, Oct 2016]

Uniprot Description

Sialomucin that may play a key role in hematopoiesis by facilitating the adhesion of CD34+ cells to the stroma and by negatively regulating CD34+CD38(lo/-) cell proliferation. Modulates the migration of umbilical cord blood CD133+ cells and this is mediated through the CXCL12/CXCR4 axis. May play an important role in prostate cancer metastasis and the infiltration of bone marrow by cancer cells. Promotes myogenesis by enhancing CXCR4-dependent cell motility. Positively regulates myoblast migration and promotes myoblast fusion into myotubes ().