Immunoprecipitation (IP)
(BMP2 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg Caco-2 Whole Cell Lysate0.5 uL anti-BMP2 mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-BMP2 mouse monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 58 kDaObserved band size: 58 kDa)
Mouse anti-Human BMP-2 Monoclonal Antibody | anti-BMP2 antibody
Anti-BMP-2 Antibody, Mouse Monoclonal
Gene Names
BMP2; BDA2; BMP2A
Reactivity
Human
Applications
Western Blot, ELISA, Immunoprecipitation
Purity
Protein A
Synonyms
BMP-2; Monoclonal Antibody; Anti-BMP-2 Antibody; Mouse Monoclonal; BMP-2 Antibody; Mouse MAb; bone morphogenetic protein 2; Anti-BDA2 Antibody; Anti-BDA2A Antibody; Anti-BMP2A Antibody; anti-BMP2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2b
Clone Number
7D10G8E2
Specificity
Human BMP-2.
No cross-reactivity in ELISA with Human BMP5 E Coli cell lysate
No cross-reactivity in ELISA with Human BMP5 E Coli cell lysate
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-BMP2 antibody
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
ELISA: 1:5000-1:10,000
IP: 0.2-1uL/mg of lysate
ELISA: 1:5000-1:10,000
IP: 0.2-1uL/mg of lysate
Immunogen
Recombinant Human BMP-2 protein
Conjugation
Unconjugated
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human BMP-2 (rh BMP-2). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunoprecipitation (IP)
(BMP2 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg Caco-2 Whole Cell Lysate0.5 uL anti-BMP2 mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-BMP2 mouse monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 58 kDaObserved band size: 58 kDa)
Immunoprecipitation (IP)
(BMP2 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg Caco-2 Whole Cell Lysate0.5 uL anti-BMP2 mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-BMP2 mouse monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 58 kDaObserved band size: 58 kDa)
Western Blot (WB)
(Anti-BMP2 mouse monoclonal antibody at 1:500 dilutionLane A: Caco2 Whole Cell LysateLane B: THP1 Whole Cell LysateLane C: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:45 kDaObserved band size:39 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-BMP2 mouse monoclonal antibody at 1:500 dilutionLane A: Caco2 Whole Cell LysateLane B: THP1 Whole Cell LysateLane C: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:45 kDaObserved band size:39 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-BMP2 antibody
BMP-2 protein, like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. BMP-2 protein is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. BMP-2 and BMP-7 are osteogenic BMPs that have been demonstrated to potently induce osteoblast differentiation in a variety of cell types. BMP-2, BMP-4 and BMP-7 are known to be of major importance in bone formation and repair. In cancerous tissues BMP-2 protein may play an important role in the progression of glioma.
Product Categories/Family for anti-BMP2 antibody
References
Jiao X, et al. (2007) Heparan sulfate proteoglycans (HSPGs) modulate BMP-2 osteogenic bioactivity in C2C12 cells. J Biol Chem. 282(2):1080-6.Michon F, et al. (2008) BMP-2 and BMP-7 play antagonistic roles in feather induction. Development 135 (16):2797-805.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
bone morphogenetic protein 2 preproprotein
NCBI Official Synonym Full Names
bone morphogenetic protein 2
NCBI Official Symbol
BMP2
NCBI Official Synonym Symbols
BDA2; BMP2A
NCBI Protein Information
bone morphogenetic protein 2; BMP-2A; bone morphogenetic protein 2A
Protein Family
NCBI Description
The protein encoded by this gene belongs to the transforming growth factor-beta (TGFB) superfamily. The encoded protein acts as a disulfide-linked homodimer and induces bone and cartilage formation. [provided by RefSeq, Jul 2008]
Research Articles on BMP2
Similar Products
Product Notes
The BMP2 (Catalog #AAA8102756) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-BMP-2 Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BMP-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:1000 ELISA: 1:5000-1:10,000 IP: 0.2-1uL/mg of lysate. Researchers should empirically determine the suitability of the BMP2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BMP-2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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