Western Blot (WB)
(Anti-mPVRL2 rabbit monoclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:58 kDaObserved band size:58 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Mouse Nectin-2 Monoclonal Antibody | anti-NECTIN2 antibody
Recombinant Anti-Nectin-2 Antibody, Rabbit Monoclonal
No cross-reactivity in ELISA with Human CD112/Nectin-2
ELISA: 1:5000-1:10,000
IP: 0.2-1uL/mg of lysate
Western Blot (WB)
(Anti-mPVRL2 rabbit monoclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:58 kDaObserved band size:58 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-mPVRL2 rabbit monoclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:58 kDaObserved band size:58 kDa(We are unsure as to the identity of these extra bands.))
Immunoprecipitation (IP)
(Mouse PVRL2 was immunoprecipitated using:Lane A:0.5 mg Raji Whole Cell Lysate0.5 uL anti-Mouse PVRL2 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse PVRL2 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 58 kDaObserved band size: 58 kDa)
Immunoprecipitation (IP)
(Mouse PVRL2 was immunoprecipitated using:Lane A:0.5 mg Raji Whole Cell Lysate0.5 uL anti-Mouse PVRL2 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse PVRL2 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 58 kDaObserved band size: 58 kDa)
NCBI and Uniprot Product Information
Uniprot Description
nectin 2: Probable cell adhesion protein. Belongs to the nectin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion; Membrane protein, integral
Cellular Component: focal adhesion; cell surface; membrane; cell; plasma membrane; integral to membrane; zonula adherens; intercellular junction
Molecular Function: identical protein binding; protein binding; protein homodimerization activity; cell adhesion molecule binding
Biological Process: establishment of mitochondrion localization; acrosome formation; sperm mitochondrion organization and biogenesis; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; positive regulation of immunoglobulin mediated immune response; spermatid nuclear differentiation; cytoskeleton organization and biogenesis; susceptibility to natural killer cell mediated cytotoxicity; virion attachment, binding of host cell surface coreceptor; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of mast cell activation; viral envelope fusion with host membrane; fertilization; cell part morphogenesis; adhesion to host; cell adhesion; homophilic cell adhesion; spermatid development
Research Articles on NECTIN2
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Product Notes
The NECTIN2 pvrl2 (Catalog #AAA8111375) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-Nectin-2 Antibody, Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Nectin-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:1000 ELISA: 1:5000-1:10,000 IP: 0.2-1uL/mg of lysate. Researchers should empirically determine the suitability of the NECTIN2 pvrl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nectin-2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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