Immunoprecipitation (IP)
(JAM-A was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate0.5 uL anti-JAM-A rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-JAM-A rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 39 kDaObserved band size: 39 kDa)
Rabbit anti-Human JAM-A/Junctional Adhesion Molecule A Polyclonal Antibody | anti-F11R antibody
Anti-JAM-A/Junctional Adhesion Molecule A Antibody, Rabbit Polyclonal
ELISA: 1:5000-1:10,000
IP: 0.2-1uL/mg of lysate
Immunoprecipitation (IP)
(JAM-A was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate0.5 uL anti-JAM-A rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-JAM-A rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 39 kDaObserved band size: 39 kDa)
Immunoprecipitation (IP)
(JAM-A was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate0.5 uL anti-JAM-A rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-JAM-A rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 39 kDaObserved band size: 39 kDa)
Western Blot (WB)
(Anti-JAM-A rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:33 kDaObserved band size:38 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-JAM-A rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:33 kDaObserved band size:38 kDa(We are unsure as to the identity of these extra bands.))
NCBI and Uniprot Product Information
NCBI Description
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established. [provided by RefSeq, Jul 2008]
Uniprot Description
JAM-A: Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly. Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus. Belongs to the immunoglobulin superfamily.
Protein type: Membrane protein, integral; Cell adhesion
Chromosomal Location of Human Ortholog: 1q21.2-q21.3
Cellular Component: microtubule cytoskeleton; tight junction; integral to membrane; plasma membrane; intercellular junction; cytoplasmic vesicle; cell junction
Molecular Function: protein binding; PDZ domain binding
Biological Process: intercellular junction assembly and maintenance; response to radiation; extracellular matrix organization and biogenesis; viral reproduction; transforming growth factor beta receptor signaling pathway; actomyosin structure organization and biogenesis; inflammatory response; cell adhesion; blood coagulation; leukocyte migration; positive regulation of blood pressure; intestinal absorption
Research Articles on F11R
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Product Notes
The F11R f11r (Catalog #AAA8101631) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-JAM-A/Junctional Adhesion Molecule An Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's JAM-A/Junctional Adhesion Molecule A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:1000 ELISA: 1:5000-1:10,000 IP: 0.2-1uL/mg of lysate. Researchers should empirically determine the suitability of the F11R f11r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JAM-A/Junctional Adhesion Molecule A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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