Rabbit anti-Human Sumo 2/3/4 Polyclonal Antibody | anti-Sumo2/3/4 antibody

Sumo 2/3/4 Rabbit pAb

Cat. Num. AAA4758266

• Gene Names: SUMO2; HSMT3; SMT3B; SUMO3; Smt3A; SMT3H2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: Sumo 2/3/4; Polyclonal Antibody; Sumo 2/3/4 Rabbit pAb; SMT3 suppressor of mif two 3 homolog 2/3/4 (S. cerevisiae); anti-Sumo2/3/4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Affinity purified
• Form/Format: -20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

• Applicable Applications for anti-Sumo2/3/4 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000; IHC: 1:100-1:300
• Immunogen: Synthetic peptide of human SUMO2/SUMO3/SUMO4
• Conjugation: Unconjugated
• Modification: Unmodified
• Cell Position: Cytoplasm, Nucleus
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 12+15%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Human ovarian cancer tissue, SKOV3 cells, Primary antibody: 860378(SUMO2/SUMO3/SUMO4 Antibody) at dilution 1/800, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 860378(SUMO2/SUMO3/SUMO4 Antibody) at dilution 1/100, on the right is treated with synthetic peptide. (Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 860378(SUMO2/SUMO3/SUMO4 Antibody) at dilution 1/100, on the right is treated with synthetic peptide. (Original magnification: ×200))

Product Categories/Family for anti-Sumo2/3/4 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 54792069
• NCBI GeneID: 6613
• NCBI Accession #: NP_008868; NP_008867; NP_001002255
• NCBI GenBank Nucleotide #: NM_006937.3
• UniProt Accession #: P61956; P55854; Q6EEV6
• Molecular Weight: 8,111 Da
• NCBI Official Full Name: small ubiquitin-related modifier 2 isoform a
• NCBI Official Synonym Full Names: small ubiquitin-like modifier 2
• NCBI Official Symbol: SUMO2
• NCBI Official Synonym Symbols: HSMT3; SMT3B; SUMO3; Smt3A; SMT3H2
• NCBI Protein Information: small ubiquitin-related modifier 2
• UniProt Protein Name: Small ubiquitin-related modifier 2
• UniProt Gene Name: SUMO2
• UniProt Synonym Gene Names: SMT3A; SMT3H2; SUMO-2; Smt3A
• UniProt Entry Name: SUMO2_HUMAN

NCBI Description

This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus have been cleaved off. Numerous pseudogenes have been reported for this gene. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

SUMO2: Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. Homotrimer (Potential). Crystal packing analysis suggests a possible trimeric assembly, of which the biological significance remains to be determined. Interacts with SAE2 and UBE2I. Covalently attached to a number of proteins. Interacts with PELP1. Interacts with USP25; the interaction sumoylates USP25. Broadly expressed. Belongs to the ubiquitin family. SUMO subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: nucleoplasm; PML body; nucleus

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: cellular protein metabolic process; protein sumoylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; post-translational protein modification