SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
14-3-3 alpha/beta Recombinant Protein | 14-3-3alpha/beta recombinant protein
14-3-3 alpha/beta Protein
Applications
Western Blot
Synonyms
14-3-3 alpha/beta; 14-3-3 alpha/beta Protein; YWHAB; HS1; GW128; KCIP-1; 14-3-3alpha/beta recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Sodium Phosphate, pH7.0, 300mM NaCl, 100mM Imidazole, 0.25mM DTT, 0.1mM PMSF, and 25% glycerol.
Applicable Applications for 14-3-3alpha/beta recombinant protein
Western Blot (WB)
Tag
HIS
Species
Mouse
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for 14-3-3alpha/beta recombinant protein
Recombinant full-length mouse 14-3-3a/b was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. The gene accession number is NM_018753.
14-3-3 alpha/beta is a member of the highly conserved 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins (1). 14-3-3 alpha/beta protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. 14-3-3-alpha/beta interacts with the TSC1-TSC2 dimer (2). The interaction required phosphorylation of TSC2 at Ser1210. Binding of 14-3-3-alpha/beta to TSC2 did not alter the interaction between TSC1 and TSC2, but it reduced the ability of the complex to inhibit phosphorylation of ribosomal protein S6 kinase impairing the ability of the complex to inhibit cell growth.
14-3-3 alpha/beta is a member of the highly conserved 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins (1). 14-3-3 alpha/beta protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. 14-3-3-alpha/beta interacts with the TSC1-TSC2 dimer (2). The interaction required phosphorylation of TSC2 at Ser1210. Binding of 14-3-3-alpha/beta to TSC2 did not alter the interaction between TSC1 and TSC2, but it reduced the ability of the complex to inhibit phosphorylation of ribosomal protein S6 kinase impairing the ability of the complex to inhibit cell growth.
Product Categories/Family for 14-3-3alpha/beta recombinant protein
References
1. Yaffe, M B. et al: The structural basis for 14-3-3: phosphopeptide binding specificity. Cell 91: 961-971, 1997.
2. Shumway, S D. et al: 14-3-3-beta binds to and negatively regulates the tuberous sclerosis complex 2 (TSC2) tumor suppressor gene product, tuberin. J. Biol. Chem. 278: 2089-2092, 2003.
2. Shumway, S D. et al: 14-3-3-beta binds to and negatively regulates the tuberous sclerosis complex 2 (TSC2) tumor suppressor gene product, tuberin. J. Biol. Chem. 278: 2089-2092, 2003.
NCBI and Uniprot Product Information
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Product Notes
The 14-3-3alpha/beta (Catalog #AAA517801) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's 14-3-3 alpha/beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the 14-3-3alpha/beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "14-3-3 alpha/beta, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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