Mouse plasminogen ELISA Kit | PLG elisa kit

Mouse Plasminogen, PLG ELISA Kit

Cat. Num. AAA910701

• Gene Names: Plg; Pg; AI649309
• Reactivity: Mouse
• Synonyms: plasminogen; Mouse Plasminogen; PLG ELISA Kit; Mouse Plasminogen (PLG) ELISA kit; RP1-81D8.1; DKFZp779M0222; plasmin; PLG elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse PLG. No significant cross-reactivity or interference between mouse PLG and analogues was observed.
• Sequence Length: 812

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Competitive
• Detection Range: 62.5 ng/ml -4000 ng/ml.
• Sensitivity: The minimum detectable dose of mouse PLG is typically less than 15.63 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLG elisa kit

Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PLG. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for PLG. The competitive inhibition reaction is launched between with pre-coated PLG and PLG in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of PLG in the samples. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 257471003
• NCBI GeneID: 18815
• NCBI Accession #: NP_032903.3
• NCBI GenBank Nucleotide #: NM_008877.3
• UniProt Accession #: P20918; Q8CIS2; Q91WJ5
• Molecular Weight: 90,808 Da
• NCBI Official Full Name: plasminogen
• NCBI Official Synonym Full Names: plasminogen
• NCBI Official Symbol: Plg
• NCBI Official Synonym Symbols: Pg; AI649309
• NCBI Protein Information: plasminogen; angiostatin; plasmin heavy chain A; plasmin light chain B
• UniProt Protein Name: Plasminogen
• Protein Family: Plasminogen
• UniProt Gene Name: Plg
• UniProt Entry Name: PLMN_MOUSE

Uniprot Description

Plasminogen: Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells. Defects in PLG are the cause of plasminogen deficiency (PLGD). PLGD is characterized by decreased serum plasminogen activity. Two forms of the disorder are distinguished: type 1 deficiency is additionally characterized by decreased plasminogen antigen levels and clinical symptoms, whereas type 2 deficiency, also known as dysplasminogenemia, is characterized by normal, or slightly reduced antigen levels, and absence of clinical manifestations. Plasminogen deficiency type 1 results in markedly impaired extracellular fibrinolysis and chronic mucosal pseudomembranous lesions due to subepithelial fibrin deposition and inflammation. The most common clinical manifestation of type 1 deficiency is ligneous conjunctivitis in which pseudomembranes formation on the palpebral surfaces of the eye progresses to white, yellow-white, or red thick masses with a wood-like consistency that replace the normal mucosa. Belongs to the peptidase S1 family. Plasminogen subfamily.

Protein type: Protease; Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide; EC 3.4.21.7

Cellular Component: extrinsic to plasma membrane; extracellular space; cell surface; extrinsic to external side of plasma membrane; intracellular membrane-bound organelle; cell; plasma membrane; extracellular region

Molecular Function: peptidase activity; protein domain specific binding; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; endopeptidase activity; apolipoprotein binding; catalytic activity; receptor binding

Biological Process: muscle maintenance; myoblast differentiation; extracellular matrix disassembly; fibrinolysis; negative regulation of angiogenesis; negative regulation of fibrinolysis; proteolysis involved in cellular protein catabolic process; hemostasis; tissue regeneration; tissue remodeling; positive regulation of fibrinolysis; blood coagulation; proteolysis