Bovine group-specific component (vitamin D binding protein) ELISA Kit | GC elisa kit

Bovine Vitamin D-binding protein, GC ELISA Kit

Cat. Num. AAA919160

• Reactivity: Bovine
• Synonyms: group-specific component (vitamin D binding protein); Bovine Vitamin D-binding protein; GC ELISA Kit; Bovine Vitamin D-binding protein (GC) ELISA kit; DBP; DBP/GC; GRD3; VDBG; VDBP; vitamin D-binding alpha-globulin; vitamin D-binding protein; GC elisa kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bovine GC. No significant cross-reactivity or interference between Bovine GC and analogues was observed.
• Sequence Length: 474

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Competitive
• Detection Range: 0.156 ug/ml-10 ug/ml
• Sensitivity: 0.039 ug/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Transport
• Protein Biological Process 3: Transport
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GC elisa kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with GC. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for GC. The competitive inhibition reaction is launched between with pre-coated GC and GC in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of GC in the samples. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 78369364
• NCBI GeneID: 530076
• NCBI Accession #: NP_001030457.1
• NCBI GenBank Nucleotide #: NM_001035380.2
• UniProt Accession #: Q3MHN5
• Molecular Weight: 53,342 Da
• NCBI Official Full Name: vitamin D-binding protein
• NCBI Official Symbol: GC
• NCBI Protein Information: vitamin D-binding protein; DBP; VDB; gc-globulin; vitamin D binding protein
• UniProt Protein Name: Vitamin D-binding protein
• Protein Family: Envelope glycoprotein
• UniProt Gene Name: GC
• UniProt Synonym Gene Names: DBP; VDB
• UniProt Entry Name: VTDB_BOVIN

Uniprot Description

Function: Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes