Rabbit plasminogen ELISA Kit | PLG elisa kit

Rabbit Plasminogen, Plg ELISA Kit

Cat. Num. AAA703242

• Reactivity: Rabbit
• Synonyms: plasminogen; Rabbit Plasminogen; Plg ELISA Kit; RP1-81D8.1; DKFZp779M0222; plasmin; PLG elisa kit

• Reactivity: Rabbit
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rabbit PLG. No significant cross-reactivity or interference between Rabbit PLG and analogues was observed.
• Sequence Length: 809

• Assay Type: Competitive
• Detection Range: 6.25ug/ml-100ug/ml
• Sensitivity: 1.56ug/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Blood Coagulation
• Protein Biological Process 3: Blood coagulation
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLG elisa kit

Introduction: Plasminogen (PLG) is a circulating zymogen that is converted to the active enzyme plasmin by cleavage of the peptide bond between Arg-560 and Val-561, which is mediated by urokinase and tissue plasminogen activator. The main function of plasmin is to dissolve fibrin blood clots. Plasmin is a serine protease that is released as plasminogen from the liver into the circulation and activated by tissue plasminogen activator (tPA), urokinase plasminogen activator (uPA), and factor XII (Hageman factor). Fibrin is a cofactor for plasminogen activation by tissue plasminogen activator. Urokinase plasminogen activator receptor (uPAR) is a cofactor for plasminogen activation by urokinase plasminogen activator. Plasmin is inactivated by alpha 2-antiplasmin, a serine protease inhibitor (serpin). Apart from fibrinolysis, plasminogen is shown to play important role in wound healing, liver repair as well as the maintenance of liver homeostasis.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to PLG has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated PLG and incubated. A competitive inhibition reaction is launched between PLG (Standards or samples) and Biotin-conjugated PLG with the pre-coated antibody specific for PLG. The more amount of PLG in samples, the less antibody bound by Biotin-conjugated PLG. Then Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of PLG in the sample. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 113205806
• NCBI GeneID: 733660
• NCBI Accession #: NP_001038055.1
• NCBI GenBank Nucleotide #: NM_001044590.2
• UniProt Accession #: P06867; Q19AZ9
• Molecular Weight: 90,615 Da
• NCBI Official Full Name: plasminogen
• NCBI Official Symbol: PLG
• NCBI Protein Information: plasminogen
• UniProt Protein Name: Plasminogen
• Protein Family: Plasminogen
• UniProt Gene Name: PLG
• UniProt Entry Name: PLMN_PIG

Uniprot Description

Function: Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells

Catalytic activity: Preferential cleavage: Lys-|-Xaa > Arg-|-Xaa; higher selectivity than trypsin. Converts fibrin into soluble products.

Enzyme regulation: Converted into plasmin by plasminogen activators, both plasminogen and its activator being bound to fibrin. Cannot be activated with streptokinase.