Typical Testing Data/Standard Curve (for reference only)
General tPA functional assay ELISA Kit | PLAT elisa kit
Active porcine tPA functional assay ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: Functionally active tPA will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. After appropriate washing steps, polyclonal anti-human tPA primary antibody binds to the captured tPA. Excess antibody is washed away and bound polyclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB is used for color development at 450nm. The amount of color development is directly proportional to the concentration of active tPA in the sample.
Background: Tissue plasminogen activator is a serine protease that catalyzes the activation of plasminogen to plasmin [1]. Clinical studies have indicated that high tPA levels may increase the risk for thrombosis [2], whereas decreased levels may cause neuronal plasticity and degeneration [3].
NCBI and Uniprot Product Information
Uniprot Description
Function: Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events
By similarity.
Catalytic activity: Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Enzyme regulation: Inhibited by SERPINA5
By similarity.
Subunit structure: Heterodimer of chain A and chain B held by a disulfide bond. Binds to fibrin with high affinity. This interaction leads to an increase in the catalytic efficiency of the enzyme due to an increase in affinity for plasminogen. Similarly, binding to heparin increases the activation of plasminogen. Binds to annexin A2, cytokeratin-8, fibronectin and laminin. Binds to mannose receptor and the low-density lipoprotein receptor-related protein (LRP1); these proteins are involved in TPA clearance. Binds LRP1B; binding is followed by internalization and degradation. Forms heterodimer with SERPINA5
By similarity.
Subcellular location: Secreted › extracellular space
By similarity.
Domain: Both FN1 and one of the kringle domains are required for binding to fibrin
By similarity.Both FN1 and EGF-like domains are important for binding to LRP1
By similarity.The FN1 domain mediates binding to annexin A2
By similarity.The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site
By similarity.
Post-translational modification: The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa
By similarity.
Sequence similarities: Belongs to the peptidase S1 family.Contains 1 EGF-like domain.Contains 1 fibronectin type-I domain.Contains 2 kringle domains.Contains 1 peptidase S1 domain.
Research Articles on PLAT
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Product Notes
The General PLAT plat (Catalog #AAA135507) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA135507 ELISA Kit recognizes General PLAT. It is sometimes possible for the material contained within the vial of "tPA functional assay, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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