Typical Testing Data/Standard Curve (for reference only)
General PAI-1 functional assay ELISA Kit | SERPINE1 elisa kit
Active rat PAI-1 functional assay ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: Functionally active PAI-1 present in plasma reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected. Unbound PAI-1 samples are washed away and an anti-PAI-1 primary antibody is added. Excess primary antibody is washed away and bound antibody, which is proportional to the original active PAI-1 present in the samples, is then reacted with the horseradish peroxidase secondary antibody. Following an additional washing step, TMB is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of active PAI-1 in the sample.
Background: Plasminogen activator inhibitor-1 (PAI-1) is a serine protease inhibitor that is an important regulator of fibrinolysis and extracellular matrix turnover [1,2,6]. PAI-1 may be important in hepatocyte growth and proliferation in vivo. Increased PAI-1 levels may increase the risk for myocardial infarction, atherosclerosis, and retenosis [3,4]. Increased PAI-1 levels may also play an important role of the development and pathogenesis of diabetic nephropathy [5]. Decreased levels may reduce thrombotic events [7].
NCBI and Uniprot Product Information
NCBI Description
mediates inhibition of fibrinolysis by inhibiting the activity of plasminogen activator; may promote neuronal survival [RGD, Feb 2006]
Uniprot Description
SERPINE1: a secreted protein that acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Belongs to the serpin family. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Plasma levels of PAI-1 and VCAM-1 together may be useful in predicting post-operative recurrence in patients with colorectal cancer.
Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted
Cellular Component: extracellular matrix; extracellular space; extracellular region
Molecular Function: serine-type endopeptidase inhibitor activity; protease binding; receptor binding
Biological Process: positive regulation of blood coagulation; wound healing; response to lipopolysaccharide; positive regulation of coagulation; decidualization; negative regulation of smooth muscle cell migration; defense response to Gram-negative bacterium; glucose homeostasis; mast cell activation; positive regulation of receptor-mediated endocytosis; positive regulation of interleukin-8 production; positive regulation of fibroblast proliferation; negative regulation of fibrinolysis; tissue regeneration; angiogenesis; regulation of receptor activity; chronological cell aging; negative regulation of cell adhesion mediated by integrin; negative regulation of cell migration; female gonad development; positive regulation of keratinocyte migration; negative regulation of proteolysis; regulation of angiogenesis; regulation of cell proliferation; response to starvation; response to reactive oxygen species; positive regulation of angiogenesis; response to hyperoxia; cellular response to insulin stimulus; response to estrogen stimulus; response to cytokine stimulus; positive regulation of inflammatory response
Research Articles on SERPINE1
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Product Notes
The General SERPINE1 serpine1 (Catalog #AAA135538) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA135538 ELISA Kit recognizes General SERPINE1. It is sometimes possible for the material contained within the vial of "PAI-1 functional assay, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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