Typical Testing Data/Standard Curve (for reference only)
General uPA functional assay ELISA Kit | PLAU elisa kit
Active mouse uPA functional assay ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: Functionally active uPA will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Only free active enzyme will react with the PAI-1 on the plate. Inactive or complexed enzyme will not be detected. After appropriate washing steps, anti-mouse uPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. The amount of color development is directly proportional to the concentration of active uPA in the sample.
Background: Urokinase plasminogen activator (uPA) is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. It is also implicated in events related to cell invasion/migration [3].
NCBI and Uniprot Product Information
Uniprot Description
uPA: Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. Defects in PLAU are the cause of Quebec platelet disorder (QPD). QPD is an autosomal dominant bleeding disorder due to a gain-of-function defect in fibrinolysis. Although affected individuals do not exhibit systemic fibrinolysis, they show delayed onset bleeding after challenge, such as surgery. The hallmark of the disorder is markedly increased PLAU levels within platelets, which causes intraplatelet plasmin generation and secondary degradation of alpha-granule proteins. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.21.73; Protease; Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted
Cellular Component: extracellular space; focal adhesion; cell surface; membrane; extracellular region
Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity
Biological Process: wound healing; smooth muscle cell migration; skeletal muscle regeneration; positive regulation of smooth muscle cell migration; proteolysis; regulation of cell proliferation; regulation of cell adhesion mediated by integrin; fibrinolysis; regulation of smooth muscle cell migration; positive regulation of cell proliferation; response to hypoxia; regulation of receptor activity; angiogenesis
Research Articles on PLAU
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Product Notes
The General PLAU plau (Catalog #AAA135514) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA135514 ELISA Kit recognizes General PLAU. It is sometimes possible for the material contained within the vial of "uPA functional assay, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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