Typical Testing Data/Standard Curve (for reference only)
General tPA functional assay ELISA Kit | PLAT elisa kit
Active rabbit tPA functional assay ELISA Kit
Reactivity
General
Synonyms
tPA functional assay; Active rabbit tPA functional assay ELISA Kit; PLAT elisa kit
Reactivity
General
Form/Format
Complete Kit
Sequence Length
564
Samples
Rabbit plasma and other biological fluids
Preparation and Storage
Store at 4 degree C. Shelf Life: 12 months from manufacture, see label for expiration date
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PLAT elisa kit
Intended Uses: This rabbit tPA activity assay is intended for the quantitative determination of active tissue plasminogen activator in rabbit plasma and other biological fluids.
Principle of the Assay: Functionally active rabbit tPA will bind to the biotinylated human PAI-1 coated on the microtiter plate. Only free active enzyme will react with the PAI-1 on the plate. A standard calibration curve is prepared using dilutions of tPA along with the samples to be measured. After appropriate washing steps, polyclonal anti-rabbit tPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound polyclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB is used for color development at 450nm. The amount of color development is directly proportional to the concentration of active tPA in the sample.
Background: Tissue plasminogen activator (tPA) is a serine protease that converts plasminogen to plasmin in the blood fibrinolytic system [1,2,3,9]. It also plays an important role in the nervous system, including the processes of neuronal migration, neurite outgrowth, and neuronal plasticity [1,2,4,7,10]. tPA has been suggested to have a role in several neuropathological conditions such as cerebral ischemia, seizures, and demyelinating diseases [1,3,5].
Principle of the Assay: Functionally active rabbit tPA will bind to the biotinylated human PAI-1 coated on the microtiter plate. Only free active enzyme will react with the PAI-1 on the plate. A standard calibration curve is prepared using dilutions of tPA along with the samples to be measured. After appropriate washing steps, polyclonal anti-rabbit tPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound polyclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB is used for color development at 450nm. The amount of color development is directly proportional to the concentration of active tPA in the sample.
Background: Tissue plasminogen activator (tPA) is a serine protease that converts plasminogen to plasmin in the blood fibrinolytic system [1,2,3,9]. It also plays an important role in the nervous system, including the processes of neuronal migration, neurite outgrowth, and neuronal plasticity [1,2,4,7,10]. tPA has been suggested to have a role in several neuropathological conditions such as cerebral ischemia, seizures, and demyelinating diseases [1,3,5].
Product Categories/Family for PLAT elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
62,726 Da
NCBI Official Full Name
tissue-type plasminogen activator
NCBI Official Symbol
PLAT
NCBI Protein Information
tissue-type plasminogen activator
UniProt Protein Name
Tissue-type plasminogen activator
Protein Family
UniProt Entry Name
Q8MKB1_RABIT
Uniprot Description
Sequence similarities: Belongs to the peptidase S1 family. RuleBase RU000360Contains 1 peptidase S1 domain. SAAS SAAS013806
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Product Notes
The General PLAT (Catalog #AAA135502) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA135502 ELISA Kit recognizes General PLAT. It is sometimes possible for the material contained within the vial of "tPA functional assay, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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