Typical Testing Data/Standard Curve (for reference only)
General PAI-1 functional assay ELISA Kit | SERPINE1 elisa kit
Active rabbit PAI-1 functional assay ELISA Kit
Gene Names
SERPINE1; PLANH1
Reactivity
General
Synonyms
PAI-1 functional assay; Active rabbit PAI-1 functional assay ELISA Kit; SERPINE1 elisa kit
Reactivity
General
Form/Format
Complete Kit
Sequence Length
402
Samples
Rabbit plasma
Preparation and Storage
Store at 4 degree C. Shelf Life: 12 months from manufacture, see label for expiration date
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SERPINE1 elisa kit
Intended Uses: Rabbit PAI-1 activity assay is intended for the quantitative determination of active plasminogen activator inhibitor type-1 in rabbit plasma.
Principle of the Assay: Functionally active PAI-1 present in plasma reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected. Unbound PAI-1 samples are washed away and an anti-PAI-1 primary antibody is added. Excess primary antibody is washed away and bound antibody, which is proportional to the original active PAI-1 present in the samples, is then reacted with the horseradish peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate solution is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of active PAI-1 in the sample.
Background: PAI-1 plays an important role in fibrinolysis and is a reported risk factor for cardiovascular disease [4]. Increased levels of PAI-1 tend to augment in the presence of proinsulin and insulin [3,5].
Principle of the Assay: Functionally active PAI-1 present in plasma reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected. Unbound PAI-1 samples are washed away and an anti-PAI-1 primary antibody is added. Excess primary antibody is washed away and bound antibody, which is proportional to the original active PAI-1 present in the samples, is then reacted with the horseradish peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate solution is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of active PAI-1 in the sample.
Background: PAI-1 plays an important role in fibrinolysis and is a reported risk factor for cardiovascular disease [4]. Increased levels of PAI-1 tend to augment in the presence of proinsulin and insulin [3,5].
Product Categories/Family for SERPINE1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
45,450 Da
NCBI Official Full Name
plasminogen activator inhibitor 1
NCBI Official Symbol
SERPINE1
NCBI Official Synonym Symbols
PLANH1
NCBI Protein Information
plasminogen activator inhibitor 1; PAI; PAI-1; serpin E1; plasminogen activator inhibitor I; endothelial plasminogen activator inhibitor
UniProt Protein Name
Plasminogen activator inhibitor 1
Protein Family
UniProt Gene Name
SERPINE1
UniProt Synonym Gene Names
PAI1; PLANH1; PAI; PAI-1
UniProt Entry Name
PAI1_PIG
Uniprot Description
Function: Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis
By similarity.
Subunit structure: Forms protease inhibiting heterodimer with TMPRSS7. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation
By similarity.
Subcellular location: Secreted.
Sequence similarities: Belongs to the serpin family.
Research Articles on SERPINE1
Similar Products
Product Notes
The General SERPINE1 serpine1 (Catalog #AAA135497) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA135497 ELISA Kit recognizes General SERPINE1. It is sometimes possible for the material contained within the vial of "PAI-1 functional assay, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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