NAGK recombinant protein

NAGK, 1-344aa, Human, His tag, E Coli

Cat. Num. AAA204125

• Gene Names: NAGK; GNK; HSA242910
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: NAGK; 1-344aa; Human; His tag; E Coli; N-acetyl-D-glucosamine kinase; GNK; HSA242910; GlcNAc kinase; NAGK recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200mM NaCl
• Concentration: 1mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAAIYGG VEGGGTRSEV LLVSEDGKIL AEADGLSTNH WLIGTDKCVE RINEMVNRAK RKAGVDPLVP LRSLGLSLSG GDQEDAGRIL IEELRDRFPY LSESYLITTD AAGSIATATP DGGVVLISGT GSNCRLINPD GSESGCGGWG HMMGDEGSAY WIAHQAVKIV FDSIDNLEAA PHDIGYVKQA MFHYFQVPDR LGILTHLYRD FDKCRFAGFC RKIAEGAQQG DPLSRYIFRK AGEMLGRHIV AVLPEIDPVL FQGKIGLPIL CVGSVWKSWE LLKEGFLLAL TQGREIQAQN FFSSFTLMKL RHSSALGGAS LGARHIGHLL PMDYSANAIA FYSYTFS
• Sequence Length: 390

• Applicable Applications for NAGK recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for NAGK recombinant protein

NAGK (N-acetyl-D-glucosamine kinase) belongs to the eukaryotic-type N-acetylglucosamine kinase family. NAGK converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. It is a prominent salvage enzyme of amino sugar metabolism in mammals. NAGK has been shown to interact with STK16 and LNX1. Also it has ManNAc kinase activity. Recombinant human NAGK protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for NAGK recombinant protein

Metabolism

References

Hinderlich S, et al. (2000) Eur J Biochem 267 (11): 3301-8.; Weihofen W.A., et al. (2006) J. Mol. Biol. 364:388-399

NCBI and Uniprot Product Information

• NCBI GI #: 295844834
• NCBI GeneID: 55577
• NCBI Accession #: NP_060037
• NCBI GenBank Nucleotide #: NM_017567.4
• UniProt Accession #: Q9UJ70; Q53HD5; Q6IA84; Q9BS29; Q9BVP0; Q9NV37; B4DLZ5
• Molecular Weight: 39.8kDa (367aa), confirmed by MALDI-TOF
• NCBI Official Full Name: N-acetyl-D-glucosamine kinase isoform 1
• NCBI Official Synonym Full Names: N-acetylglucosamine kinase
• NCBI Official Symbol: NAGK
• NCBI Official Synonym Symbols: GNK; HSA242910
• NCBI Protein Information: N-acetyl-D-glucosamine kinase
• UniProt Protein Name: N-acetyl-D-glucosamine kinase
• Protein Family: N-acetyl-D-glucosamine kinase
• UniProt Gene Name: NAGK
• UniProt Synonym Gene Names: N-acetylglucosamine kinase

NCBI Description

This gene encodes a member of the N-acetylhexosamine kinase family. The encoded protein catalyzes the conversion of N-acetyl-D-glucosamine to N-acetyl-D-glucosamine 6-phosphate, and is the major mammalian enzyme which recovers amino sugars. [provided by RefSeq, Nov 2011]

Uniprot Description

Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway: although human is not able to catalyze formation of Neu5Gc due to the inactive CMAHP enzyme, Neu5Gc is present in food and must be degraded. Also has ManNAc kinase activity.