Mouse GAPDH Monoclonal Antibody | anti-GAPDH antibody

GAPDH Antibody

Cat. Num. AAA9217031

• Gene Names: GAPDH; G3PD; GAPD; HEL-S-162eP
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Synonyms: GAPDH; Monoclonal Antibody; GAPDH Antibody; Glyceraldehyde-3-phosphate dehydrogenase; 1.2.1.12; Peptidyl-cysteine S-nitrosylase GAPDH; 2.6.99.-; GAPD; anti-GAPDH antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1,k
• Specificity: This GAPDH antibody is generated from a mouse immunized with a recombinant protein between 43-335 amino acids from human GAPDH.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Sequence Length: 293

• Applicable Applications for anti-GAPDH antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB:~~1:8000
• Subtitle: Purified Mouse Monoclonal Antibody (Mab)
• Cellular Location: Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Cytoplasm, cytoskeleton. Note=Translocates to the nucleus following S- nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
• Antigen Type: Recombinant Protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-GAPDH Antibody at 1:8000 dilutionLane 1: C6 whole cell lysateLane 2: mouse brain lysateLane 3: NIH/3T3 whole cell lysateLane 4: Hela whole cell lysateLane 5: Jurkat whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(All lanes : Anti-GAPDH Antibody at 1:8000 dilutionLane 1: Hela whole cell lysateLane 2: Jurkat whole cell lysateLane 3: A549 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(All lanes : Anti-GAPDH Antibody at 1:8000 dilutionLane 1: Hela whole cell lysateLane 2: Jurkat whole cell lysateLane 3: A549 whole cell lysateLane 4: C6 whole cell lysateLane 5: NIH/3T3 whole cell lysateLane 6: mouse brain lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-GAPDH antibody

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon- activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

Product Categories/Family for anti-GAPDH antibody

Stem Cells; Signal Transduction; Neuroscience; Microbiology; Metabolism; Cancer; Antibody Collections; Primary Antibodies; Crown Antibodies; Loading Control

References

Hanauer A.,et al.EMBO J. 3:2627-2633 (1984). Arcari P.,et al.Nucleic Acids Res. 12:9179-9189 (1984). Tso J.Y.,et al.Nucleic Acids Res. 13:2485-2502 (1985). Tokunaga K.,et al.Cancer Res. 47:5616-5619 (1987). Allen R.W.,et al.J. Biol. Chem. 262:649-653 (1987).

NCBI and Uniprot Product Information

• NCBI GI #: 378404908
• NCBI GeneID: 2597
• NCBI Accession #: NP_001243728.1
• NCBI GenBank Nucleotide #: NM_001256799.2
• UniProt Accession #: P04406; P00354; Q53X65; E7EUT4
• Molecular Weight: 36053 Da
• NCBI Official Full Name: glyceraldehyde-3-phosphate dehydrogenase isoform 2
• NCBI Official Synonym Full Names: glyceraldehyde-3-phosphate dehydrogenase
• NCBI Official Symbol: GAPDH
• NCBI Official Synonym Symbols: G3PD; GAPD; HEL-S-162eP
• NCBI Protein Information: glyceraldehyde-3-phosphate dehydrogenase
• UniProt Protein Name: Glyceraldehyde-3-phosphate dehydrogenase
• UniProt Gene Name: GAPDH
• UniProt Synonym Gene Names: GAPD; GAPDH
• UniProt Entry Name: G3P_HUMAN

NCBI Description

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

Uniprot Description

GAPDH: a multifunctional enzyme with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities. A key glycolytic enzyme that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. An important enzyme for energy metabolism, and the production of ATP and pyruvate through anaerobic glycolysis in the cytoplasm. Additionally, it participates in apoptosis, membrane trafficking, iron metabolism, nuclear activities and receptor mediated cell signaling. Its subcellular localization changes reflecting its multiple activities. Is cytosolic, but is also localized in the membrane, the nucleus, polysomes, the ER and the Golgi. Participates in transcription, RNA transport, DNA replication and apoptosis. S-nitrosylation on Cys-152 following apoptotic stimulates its interaction with SIAH2, which in turn moderates its translocation into the nucleus. Mediates cysteine S-nitrosylation of nuclear target proteins including SIRT1, HDAC2 and DNA-PK. Deregulated in lung cancer, renal cancer, breast cancer, gastric cancer, glioma, liver cancer, colorectal cancer, melanoma, prostatic cancer, pancreatic cancer and bladder cancer. Its increased expression and enzymatic activity is associated with cell proliferation and tumorigenesis, Oxidative stress impairs GAPDH catalytic activity and leads to cellular aging and apoptosis. In experimental animal models, injection of GAPDH antagonists induces apoptosis and blocks Hep3B tumor progression, suggesting a therapeutic potential of targeting GAPDH in human hepatocellular carcinoma

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 1.2.1.12; Oxidoreductase

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; lipid particle; membrane; microtubule cytoskeleton; nuclear membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; ribonucleoprotein complex; vesicle

Molecular Function: glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity; identical protein binding; microtubule binding; NAD binding; NADP binding; protein binding

Biological Process: gluconeogenesis; microtubule cytoskeleton organization and biogenesis; negative regulation of translation; neuron apoptosis; protein stabilization; regulation of macroautophagy