FLISA
(FLISA plate was coated with purified goat IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Mouse Anti-Goat IgG Fc-FITC .)
IgG secondary antibody
Mouse Anti-Goat IgG Fc-FITC
Reactivity
Goat
Applications
ELISA, Western Blot, Immunohistochemistry
Synonyms
IgG; Monoclonal Secondary Antibody; Mouse Anti-Goat IgG Fc-FITC; IgG secondary antibody
Host
Mouse
Reactivity
Goat
Clonality
Monoclonal
Isotype
Mouse(BALB/c) IgG2a kappa
Clone Number
SB115d
Specificity
Goat IgG Fc
Minimal reactivity to goat IgG Fab and sheep, rabbit, human, monkey, rat, hamster, canine, and swine IgG
Minimal reactivity to goat IgG Fab and sheep, rabbit, human, monkey, rat, hamster, canine, and swine IgG
Form/Format
Phosphate buffered saline containing <0.1% sodium azide
Concentration
0.5mg/mL (varies by lot)
Applicable Applications for IgG secondary antibody
ELISA (EIA), Fluorescence-Linked Immunosorbent Assay (FLISA), Western Blot (WB), Immunohistochemistry-Frozen (IHC-F) Sections
Application Notes
Applications for relevant formats of this clone include-
ELISA- Quality tested 1
FLISA- Quality tested
Western Blot- Reported in literature 2
Immunohistochemistry-Frozen Sections 3
ELISA- Quality tested 1
FLISA- Quality tested
Western Blot- Reported in literature 2
Immunohistochemistry-Frozen Sections 3
Immunogen
Pooled goat IgG
Conjugation
FITC (Fluorescein)
Preparation and Storage
Store at 2-8 degree C. Avoid exposure to light. Please refer to product specific SDS
FLISA
(FLISA plate was coated with purified goat IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Mouse Anti-Goat IgG Fc-FITC .)
FLISA
(FLISA plate was coated with purified goat IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Mouse Anti-Goat IgG Fc-FITC .)
References
1. Tang J, Sun X, Yang H, Zhang B, Li Z, Lin Z, et al. Well-oriented ZZ-PS-tag with high Fc-binding onto polystyrene surface for controlled immobilization of capture antibodies. Anal Chim Acta. 2013;776:74-8. (ELISA)
2. Mohapatra A, Van Dyken SJ, Schneider C, Nussbaum JC, Liang H, Locksley RM. Group 2 innate lymphoid cells utilize the IRF4-IL-9 module to coordinate epithelial cell maintenance of lung homeostasis. Mucosal Immunol. 2016;9:275-86. (WB)
3. SouthernBiotech published data (IHC-FS)
2. Mohapatra A, Van Dyken SJ, Schneider C, Nussbaum JC, Liang H, Locksley RM. Group 2 innate lymphoid cells utilize the IRF4-IL-9 module to coordinate epithelial cell maintenance of lung homeostasis. Mucosal Immunol. 2016;9:275-86. (WB)
3. SouthernBiotech published data (IHC-FS)
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Product Notes
The IgG (Catalog #AAA679411) is a Secondary Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Goat IgG Fc-FITC reacts with Goat and may cross-react with other species as described in the data sheet. AAA Biotech's IgG can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Fluorescence-Linked Immunosorbent Assay (FLISA), Western Blot (WB), Immunohistochemistry-Frozen (IHC-F) Sections. Applications for relevant formats of this clone include- ELISA- Quality tested 1 FLISA- Quality tested Western Blot- Reported in literature 2 Immunohistochemistry-Frozen Sections 3. Researchers should empirically determine the suitability of the IgG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG, Monoclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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