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Western Blot (WB) (Western blot analysis of hNAGK-G315 (RB05452) in 293 cell line lysates (35ug/lane).NAGK (arrow) was detected using the purified Pab.)

Rabbit anti-Human, mouse NAGK Polyclonal Antibody | anti-NAGK antibody

NAGK Antibody (C-term)

Gene Names
NAGK; GNK; HSA242910
Reactivity
Human, mouse
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NAGK; Polyclonal Antibody; NAGK Antibody (C-term); N-acetyl-D-glucosamine kinase; N-acetylglucosamine kinase; GlcNAc kinase; anti-NAGK antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NAGK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300-330 amino acids from the C-terminal region of human NAGK.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
300-330
Sequence Length
390
Applicable Applications for anti-NAGK antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of hNAGK-G315 (RB05452) in 293 cell line lysates (35ug/lane).NAGK (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of hNAGK-G315 (RB05452) in 293 cell line lysates (35ug/lane).NAGK (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of hNAGK-G315 (RB05452) in mouse stomach tissue lysates (35ug/lane).NAGK (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of hNAGK-G315 (RB05452) in mouse stomach tissue lysates (35ug/lane).NAGK (arrow) was detected using the purified Pab.)
Related Product Information for anti-NAGK antibody
N-acetylglucosamine kinase (NAGK) converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. NAGK belongs to the group of N-acetylhexosamine kinases and is a prominent salvage enzyme of amino sugar metabolism in mammals. The predicted 344-amino acid NAGK protein contains the 5 sequence motifs necessary for the binding of ATP by sugar kinases. NAGK shares 91.6% amino acid similarity with mouse Nagk, for which enzyme activity is detectable in all mouse tissues examined, with highest enzymatic activity in testis. It is hypothesized that NAGK has a general role in the catabolic pathways of GlcNAc as well as of ManNAc.
Product Categories/Family for anti-NAGK antibody
References
Hinderlich, S., et al., Eur. J. Biochem. 267(11):3301-3308 (2000).
Lowes, W., et al., Biochim. Biophys. Acta 1379(1):134-142 (1998).
Weidanz, J.A., et al., Br. J. Haematol. 95(4):645-653 (1996).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37376
NCBI Official Full Name
N-acetyl-D-glucosamine kinase
NCBI Official Synonym Full Names
N-acetylglucosamine kinase
NCBI Official Symbol
NAGK
NCBI Official Synonym Symbols
GNK; HSA242910
NCBI Protein Information
N-acetyl-D-glucosamine kinase
UniProt Protein Name
N-acetyl-D-glucosamine kinase
UniProt Gene Name
NAGK
UniProt Synonym Gene Names
N-acetylglucosamine kinase
UniProt Entry Name
NAGK_HUMAN

NCBI Description

This gene encodes a member of the N-acetylhexosamine kinase family. The encoded protein catalyzes the conversion of N-acetyl-D-glucosamine to N-acetyl-D-glucosamine 6-phosphate, and is the major mammalian enzyme which recovers amino sugars. [provided by RefSeq, Nov 2011]

Uniprot Description

NAGK: Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Also has ManNAc kinase activity. Belongs to the eukaryotic-type N-acetylglucosamine kinase family.

Protein type: Carbohydrate Metabolism - amino sugar and nucleotide sugar; Kinase, other; EC 2.7.1.59

Chromosomal Location of Human Ortholog: 2p13.3

Molecular Function: protein binding; N-acetylglucosamine kinase activity; ATP binding; N-acylmannosamine kinase activity

Biological Process: N-acetylneuraminate catabolic process; N-acetylglucosamine metabolic process; carbohydrate phosphorylation; N-acetylmannosamine metabolic process

Research Articles on NAGK

Similar Products

Product Notes

The NAGK nagk (Catalog #AAA9201441) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 300-330. The NAGK Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NAGK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the NAGK nagk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NAGK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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