Western Blot (WB)

MuRF1 recombinant protein

MuRF1 Recombinant Protein

Applications

Western Blot

Synonyms

MuRF1; MuRF1 Recombinant Protein; MuRFR; MuRF1 recombinant protein

Ordering
Cat. Num. AAA474430 For Research Use Only!

Host

Lysate

Form/Format

MuRF1 recombinant protein lysate is supplied at a concentration of 10ng/ul in electrophoresis sample buffer (62.5mM Tris pH6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% beta-mercaptoethanol).

Applicable Applications for MuRF1 recombinant protein

Western Blot (WB)

Application Notes

WB: 10ul/lane

Preparation and Storage

Store at -20 degree C. Do not boil or dilute. Stable for 1 year.

Western Blot (WB)

(Western blot analysis of human full length MuRF1 recombinant protein. The blot was probed with mouse monoclonal MuRF1 (C-terminal region) at 1:250 (lane 1) and 1:1000 (lane 2) and rabbit polyclonal MuRF1 (C-terminal region) at 1:1000 (lanes 3) and 1:4000 (lane 4).)

Related Product Information for MuRF1 recombinant protein

Muscle proteolysis is regulated by the ATP-dependent ubiquitin-proteasome system. This system involves ubiquitination of specific proteins, leading to recognition and degradation by the 26S proteasome complex. Ubiquitination requires interactions with ubiquitin related proteins, ubiquitin-activating (E1), ubiquitin-conjugating (E2) and ubiquitin-ligating enzymes (E3) known as ligases. Two muscle specific ubiquitin ligases have been identified, muscle ring finger 1 (MuRF-1) and Atrogin 1. Both ligases are regulated by the Akt1/FOXO1 signaling pathway, and both proteins have been shown to be upregulated prior to the onset of atrophy in multiple models of muscle wasting, including disuse and cachexia. MuRF1 is also known as TRIM63, SMRZ, and RNF28, and its expression is upregulated after TNFalpha treatment in C2C12 cells and muscle tissue, while localization of MuRF1 protein has been observed in the cytoplasm and nucleus of cells.Human recombinant MuRF1 is a 40kDa protein that includes the full length sequence along with an N-terminal His tag. The MuRF1 protein is detected by rabbit polyclonal anti-MuRF1 .

References

Leger, B. et al. (2006) J Physiol. 576(3):923.
Bodine, S.C. (2001) Science. 294(5547):1704.
Dai, K.S. & Liews, C.C. (2001) J Biol. Chem. 276(26):23992.

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Product Notes

The MuRF1 (Catalog #AAA474430) is a Recombinant Protein produced from Lysate and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MuRF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 10ul/lane. Researchers should empirically determine the suitability of the MuRF1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MuRF1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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MuRF1 recombinant protein