Mouse MuRF1 Monoclonal Antibody | anti-MuRF1 antibody

MuRF1 (C-terminal region) M316 Antibody

Cat. Num. AAA474442

• Gene Names: TRIM63; IRF; SMRZ; MURF1; MURF2; RNF28
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: Protein G chromatography
• Synonyms: MuRF1; Monoclonal Antibody; MuRF1 (C-terminal region) M316 Antibody; MuRF1 (C-terminal region); MuRF mAb; anti-MuRF1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: M316
• Specificity: This mouse monoclonal antibody was purified with protein G chromatography. The antibody detects a full length human MuRF1 recombinant protein on SDS-PAGE immunoblots.
• Purity/Purification: Protein G chromatography
• Form/Format: Mouse monoclonal purified with protein G chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-MuRF1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA: 1:1000; WB: 1:250
• Immunogen: Clone M316 was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal half of human MuRF1. This peptide sequence is highly conserved in rat and mouse MuRF1, and has 50% homology to MuRF2 (TRIM-55).
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot analysis of human full length MuRF1 recombinant protein. The blot was probed with mouse monoclonal MuRF1 (C-terminal region) at 1:250 (lane 1) and 1:1000 (lane 2) and rabbit polyclonal MuRF1 (C-terminal region) at 1:1000 (lanes 3) and 1:4000 (lane 4).)

Related Product Information for anti-MuRF1 antibody

Muscle proteolysis is regulated by the ATP-dependent ubiquitin-proteasome system. This system involves ubiquitination of specific proteins, leading to recognition and degradation by the 26S proteasome complex. Ubiquitination requires interactions with ubiquitin related proteins, ubiquitin-activating (E1), ubiquitin-conjugating (E2) and ubiquitin-ligating enzymes (E3) known as ligases. Two muscle specific ubiquitin ligases have been identified, muscle ring finger 1 (MuRF-1) and Atrogin 1. Both ligases are regulated by the Akt1/FOXO1 signaling pathway, and both proteins have been shown to be upregulated prior to the onset of atrophy in multiple models of muscle wasting, including disuse and cachexia. MuRF1 is also known as TRIM63, SMRZ, and RNF28, and its expression is upregulated after TNFalpha treatment in C2C12 cells and muscle tissue, while localization of MuRF1 protein has been observed in the cytoplasm and nucleus of cells.

References

Leger, B. et al. (2006) J Physiol. 576(3):923.
Bodine, S.C. (2001) Science. 294(5547):1704.
Dai, K.S. & Liews, C.C. (2001) J Biol. Chem. 276(26):23992.

NCBI and Uniprot Product Information

• NCBI GI #: 21362898
• NCBI GeneID: 84676
• UniProt Accession #: Q969Q1; Q5T2I1; Q96BD3; Q96KD9; Q9BYV4; B4DN95
• Molecular Weight: 353
• NCBI Official Full Name: E3 ubiquitin-protein ligase TRIM63
• NCBI Official Synonym Full Names: tripartite motif containing 63, E3 ubiquitin protein ligase
• NCBI Official Symbol: TRIM63
• NCBI Official Synonym Symbols: IRF; SMRZ; MURF1; MURF2; RNF28
• NCBI Protein Information: E3 ubiquitin-protein ligase TRIM63; ring finger protein 28; iris ring finger protein; muscle specific ring finger protein 2; muscle-specific RING finger protein 1; tripartite motif-containing protein 63; striated muscle RING zinc finger protein
• UniProt Protein Name: E3 ubiquitin-protein ligase TRIM63
• UniProt Gene Name: TRIM63
• UniProt Synonym Gene Names: IRF; MURF1; RNF28; SMRZ; MuRF-1; MuRF1
• UniProt Entry Name: TRI63_HUMAN

NCBI Description

This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that localizes to the Z-line and M-line lattices of myofibrils. This protein plays an important role in the atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy chain proteins, myosin light chain, myosin binding protein, and for muscle-type creatine kinase. [provided by RefSeq, Feb 2012]

Uniprot Description

MURF1: E3 ubiquitin ligase. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric- associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.

Protein type: EC 6.3.2.-; Ligase; EC 6.3.2.19; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1p34-p33

Cellular Component: microtubule; cytoplasm; M band; Z disc; nucleus

Molecular Function: signal transducer activity; protein binding; zinc ion binding; ubiquitin-protein ligase activity; titin binding; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; skeletal muscle atrophy; muscle contraction; regulation of gene expression; protein ubiquitination; response to electrical stimulus involved in regulation of muscle adaptation; signal transduction