Western Blot (WB)
((0.5ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat DYRK1A Polyclonal Antibody | anti-DYRK1A antibody
Goat anti-DYRK1A Antibody
Western Blot: Approx 80kDa band observed in lysates of cell line K562 (calculated MW of 85.6kDa according to NP_001387.2). Recommended concentration: 0.5-2ug/ml.
Western Blot (WB)
((0.5ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. Alternative splicing of this gene generates several transcript variants differing from each other either in the 5' UTR or in the 3' coding region. These variants encode at least five different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
DYRK1A: a dual-specificity protein kinase of the DYRK family. Contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. May play a significant role in a pathway regulating cell proliferation and may be involved in brain development. Its gene is localized in the Down syndrome (DS) critical region of chromosome 21, and is a strong candidate gene for learning defects associated with Down syndrome. Overexpression in mice gives rise to neurological abnormalities similar to those observed in DS. Five splice-variant isoforms have been described.
Protein type: Kinase, protein; Protein kinase, dual-specificity (non-receptor); EC 2.7.12.2; Protein kinase, CMGC; CMGC group; DYRK family; Dyrk1 subfamily
Chromosomal Location of Human Ortholog: 21q22.13
Cellular Component: nuclear speck; nucleoplasm; nucleus; ribonucleoprotein complex
Molecular Function: ATP binding; identical protein binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein kinase activity; protein self-association; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity; protein-tyrosine kinase activity; tau protein binding
Biological Process: circadian rhythm; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of nuclear mRNA splicing, via spliceosome; nervous system development; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of alternative nuclear mRNA splicing, via spliceosome
Disease: Mental Retardation, Autosomal Dominant 7
Research Articles on DYRK1A
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Product Notes
The DYRK1A dyrk1a (Catalog #AAA423127) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-DYRK1A Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's DYRK1A can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 80kDa band observed in lysates of cell line K562 (calculated MW of 85.6kDa according to NP_001387.2). Recommended concentration: 0.5-2ug/ml. Researchers should empirically determine the suitability of the DYRK1A dyrk1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PHSHQYSDRR QPN. It is sometimes possible for the material contained within the vial of "DYRK1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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