Human Coagulation Factor V (F5) RTU ELISA Kit | F5 rtu elisa kit

Human Coagulation Factor V (F5) ELISA Kit

Cat. Num. AAA459147

• Gene Names: F5; FVL; PCCF; THPH2; RPRGL1
• Reactivity: Human
• Synonyms: Coagulation Factor V (F5); Human Coagulation Factor V (F5) ELISA Kit; FV; FVL; PCCF; Proaccelerin; Labile Factor; Factor V Leiden; Accelerator Globulin; Activated protein C cofactor; labile factor; F5 rtu elisa kit

• Reactivity: Human
• Sequence Length: 2224

• Assay Type: Sandwich
• Samples: Plasma.
• Detection Range: 62.5-4000pg/mL
• Sensitivity: 23.9pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for F5 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to F5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to F5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain F5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of F5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 105990535
• NCBI GeneID: 2153
• NCBI Accession #: NP_000121.2
• NCBI GenBank Nucleotide #: NM_000130.4
• UniProt Accession #: P12259; Q14285; Q2EHR5; Q5R346; Q5R347; Q6UPU6; Q8WWQ6; A8K6E8
• Molecular Weight: 251,703 Da
• NCBI Official Full Name: coagulation factor V preproprotein
• NCBI Official Synonym Full Names: coagulation factor V
• NCBI Official Symbol: F5
• NCBI Official Synonym Symbols: FVL; PCCF; THPH2; RPRGL1
• NCBI Protein Information: coagulation factor V
• UniProt Protein Name: Coagulation factor V
• Protein Family: Coagulation factor
• UniProt Gene Name: F5

NCBI Description

This gene encodes an essential cofactor of the blood coagulation cascade. This factor circulates in plasma, and is converted to the active form by the release of the activation peptide by thrombin during coagulation. This generates a heavy chain and a light chain which are held together by calcium ions. The activated protein is a cofactor that participates with activated coagulation factor X to activate prothrombin to thrombin. Defects in this gene result in either an autosomal recessive hemorrhagic diathesis or an autosomal dominant form of thrombophilia, which is known as activated protein C resistance. [provided by RefSeq, Oct 2008]

Uniprot Description

Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.