Western Blot (WB)
(Western blot of human recombinant SK2 (lanes 1-4). The blots were untreated (lane 1 & 3) or treated with lambda phosphatase (lane 2 & 4), then probed with anti-SK2 (N-terminal region) (lanes 1 & 2) or anti-SK2 (Thr-578) (lanes 3 & 4).)
Sphingosine Kinase 2 Recombinant Protein | SPHK2X recombinant protein
Sphingosine Kinase 2 Recombinant Protein
Applications
Western Blot
Synonyms
Sphingosine Kinase 2; Sphingosine Kinase 2 Recombinant Protein; SPHK2X recombinant protein
Host
Lysate
Form/Format
SK2 recombinant protein lysate is supplied at a concentration of 2ng/ul in electrophoresis sample buffer (62.5mM Tris pH6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% beta-mercaptoethanol).
Applicable Applications for SPHK2X recombinant protein
Western Blot (WB)
Application Notes
WB: 20ul/lane
Preparation and Storage
Store at -20 degree C. Do not boil or dilute. Stable for 1 year.
Western Blot (WB)
(Western blot of human recombinant SK2 (lanes 1-4). The blots were untreated (lane 1 & 3) or treated with lambda phosphatase (lane 2 & 4), then probed with anti-SK2 (N-terminal region) (lanes 1 & 2) or anti-SK2 (Thr-578) (lanes 3 & 4).)
Western Blot (WB)
(Western blot of human recombinant SK2 (lanes 1-4). The blots were untreated (lane 1 & 3) or treated with lambda phosphatase (lane 2 & 4), then probed with anti-SK2 (N-terminal region) (lanes 1 & 2) or anti-SK2 (Thr-578) (lanes 3 & 4).)
Related Product Information for SPHK2X recombinant protein
Sphingosine Kinase (SK) catalyzes the phosphorylation of the lipid sphingosine leading to S1P-mediated cell proliferation, survival, motility and differentiation. Two isoforms of SK have been identified, SK1 and SK2. The mRNA for both of these isoforms is widely expressed with SK1 expression highest in brain, heart, kidney, thymus, spleen and lung, while SK2 is highest in kidney and liver. Regulation of SK1 and SK2 activity may occur through phosphorylation. SK1 is phosphorylated at Ser-225 by ERK leading to increased activity and translocation to the plasma membrane. SK2 is phosphorylated in response to EGF, PKC activators, and phorbol esters. ERK1 can phosphorylate both Ser-351 and Thr-578, and non-phosphorylatable mutants of these sites suppress ERK1-mediated chemotaxis.
Human recombinant SK2 is a full length protein with an N-terminal GST fusion protein. The protein is detected by rabbit polyclonal anti-SK2 (N-terminal region) antibody and anti-SK2 (Thr-578) phospho-specific antibody .
Human recombinant SK2 is a full length protein with an N-terminal GST fusion protein. The protein is detected by rabbit polyclonal anti-SK2 (N-terminal region) antibody and anti-SK2 (Thr-578) phospho-specific antibody .
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Product Notes
The SPHK2X (Catalog #AAA474580) is a Recombinant Protein produced from Lysate and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Sphingosine Kinase 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 20ul/lane. Researchers should empirically determine the suitability of the SPHK2X for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sphingosine Kinase 2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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