Dot Blot (DB)
(Dot blot of 5-Methylcytosine (5-mC) antibody (rAb).Dot blot of double stranded DNA using 5-Methylcytosine (5-mC) antibody (RM231). The membrane was pre-spotted with 50, 5, and 0.5 ng/dot of double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA. The pre-spotted membrane was then blotted with 5-Methylcytosine (5-mC) antibody at 0.5 ug/ml.)
Mouse anti-Human 5-Methylcytosine Antibody
5-Methylcytosine (5-mC) antibody (rAb)(Clone RM231)
Reactivity
Human
Applications
Dot Blot, Immunocytochemistry, Immunoprecipitation
Purity
Protein A Chromatography
Synonyms
5-Methylcytosine; 5-Methylcytosine (5-mC) antibody (rAb)(Clone RM231); anti-5-Methylcytosine antibody
Host
Mouse
Reactivity
Human
Isotype
IgG
Clone Number
RM231
Purity/Purification
Protein A Chromatography
Form/Format
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-5-Methylcytosine antibody
Dot Blot (DB), Immunocytochemistry (ICC), Methyl DNA Immunoprecipitation (MeDIP)
Application Notes
DB: 0.5-2ug/ml
ICC: 0.5-2ug/ml
MeDIP: 0.2-2ug/ml
ICC: 0.5-2ug/ml
MeDIP: 0.2-2ug/ml
Antibody Type
Recombinant Antibody
Immunogen
BSA-conjugated 5-methylcytosine.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Dot Blot (DB)
(Dot blot of 5-Methylcytosine (5-mC) antibody (rAb).Dot blot of double stranded DNA using 5-Methylcytosine (5-mC) antibody (RM231). The membrane was pre-spotted with 50, 5, and 0.5 ng/dot of double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA. The pre-spotted membrane was then blotted with 5-Methylcytosine (5-mC) antibody at 0.5 ug/ml.)
Dot Blot (DB)
(Dot blot of 5-Methylcytosine (5-mC) antibody (rAb).Dot blot of double stranded DNA using 5-Methylcytosine (5-mC) antibody (RM231). The membrane was pre-spotted with 50, 5, and 0.5 ng/dot of double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA. The pre-spotted membrane was then blotted with 5-Methylcytosine (5-mC) antibody at 0.5 ug/ml.)
Testing Data
(MeDIP of 5-Methylcytosine (5-mC) antibody (rAb).MeDIP was performed using 5-Methylcytosine (5-mC) antibody (RM231) at a 2:1 DNA:Ab ratio. 1 ng of unmethylated, 5-Methylcytosine (5-mC) or 5-Hydroxymethylcytosine (5-hmC) DNA standard (897 bp) was spiked in 1 ug of genomic DNA isolated from HeLa cells as the control. Realtime PCR was then performed to determine the capture of DNA standard as in % of input.)
Testing Data
(MeDIP of 5-Methylcytosine (5-mC) antibody (rAb).MeDIP was performed using 5-Methylcytosine (5-mC) antibody (RM231) at a 2:1 DNA:Ab ratio. 1 ng of unmethylated, 5-Methylcytosine (5-mC) or 5-Hydroxymethylcytosine (5-hmC) DNA standard (897 bp) was spiked in 1 ug of genomic DNA isolated from HeLa cells as the control. Realtime PCR was then performed to determine the capture of DNA standard as in % of input.)
Immunofluorescence (IF)
(Immunofluorescence stain of 5-Methylcytosine (5-mC) antibody (rAb).HeLa cells were stained using 5-Methylcytosine (5-mC) antibody (RM231) antibody at 1 ug/ml (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).)
Immunofluorescence (IF)
(Immunofluorescence stain of 5-Methylcytosine (5-mC) antibody (rAb).HeLa cells were stained using 5-Methylcytosine (5-mC) antibody (RM231) antibody at 1 ug/ml (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).)
Related Product Information for anti-5-Methylcytosine antibody
Short Description: Our 5-Methylcytosine (5-mC) antibody (rAb)(Clone RM231) was raised in a Rabbit host. It has been validated for use in Dot blot, Immunocytochemistry and Methyl DNA Immunoprecipitation; it has been shown to react with Human samples, but the sequence is not species specific so it should react with a wide range of sample types.
Background: 5-Methylcytosine (5-Methylcytidine) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease.
Background: 5-Methylcytosine (5-Methylcytidine) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease.
Product Categories/Family for anti-5-Methylcytosine antibody
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Product Notes
The 5-Methylcytosine (Catalog #AAA389510) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The 5-Methylcytosine (5-mC) antibody (rAb)(Clone RM231) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's 5-Methylcytosine can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Immunocytochemistry (ICC), Methyl DNA Immunoprecipitation (MeDIP). DB: 0.5-2ug/ml ICC: 0.5-2ug/ml MeDIP: 0.2-2ug/ml. Researchers should empirically determine the suitability of the 5-Methylcytosine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "5-Methylcytosine, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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