Dot Blot (DB)
(Dot blot analysis was used to confirm the specificity of 5-Formylcytosine antibody for 5-Formylcytidine Various BSA conjugated nucleosides were spotted onto PVDF membrane and probed with the antibody at 1:10,000. Column 1: 5-fC. Column. 2: 5-caC. Column. 3: 5-hmC. Column. 4: Cytidine. Column. 5: 5-mC.)
Rabbit 5-Formylcytosine Polyclonal Antibody
5-Formylcytosine (5-fC) antibody (pAb)
Reactivity
5-Formylcytosine
Applications
Dot Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Synonyms
5-Formylcytosine; Polyclonal Antibody; 5-Formylcytosine (5-fC) antibody (pAb); anti-5-Formylcytosine antibody
Host
Rabbit
Reactivity
5-Formylcytosine
Clonality
Polyclonal
Form/Format
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-5-Formylcytosine antibody
Dot Blot (DB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
Applications Validated:
DB: 1:5,000 dilution
DB: 1:5,000 dilution
Conjugation
Methylated
Immunogen
This 5-Formylcytosine antibody was raised against 5-formylcytidine conjugated to KLH and recognizes 5-formylcytosine.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Dot Blot (DB)
(Dot blot analysis was used to confirm the specificity of 5-Formylcytosine antibody for 5-Formylcytidine Various BSA conjugated nucleosides were spotted onto PVDF membrane and probed with the antibody at 1:10,000. Column 1: 5-fC. Column. 2: 5-caC. Column. 3: 5-hmC. Column. 4: Cytidine. Column. 5: 5-mC.)
Dot Blot (DB)
(Dot blot analysis was used to confirm the specificity of 5-Formylcytosine antibody for 5-Formylcytidine Various BSA conjugated nucleosides were spotted onto PVDF membrane and probed with the antibody at 1:10,000. Column 1: 5-fC. Column. 2: 5-caC. Column. 3: 5-hmC. Column. 4: Cytidine. Column. 5: 5-mC.)
Related Product Information for anti-5-Formylcytosine antibody
Short Description: Our 5-Formylcytosine (5-fC) antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry and Western blot; the sequence is not species specific so it should react with a wide range of sample types.
Background: DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC into 5-formylcytosine and 5-carboxylcytosine. An increase in levels of 5-formylcytosine and 5-carboxylcytosine are detected in the mouse male pronucleus following fertilization, which is gradually diluted by DNA replication.
Background: DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC into 5-formylcytosine and 5-carboxylcytosine. An increase in levels of 5-formylcytosine and 5-carboxylcytosine are detected in the mouse male pronucleus following fertilization, which is gradually diluted by DNA replication.
Product Categories/Family for anti-5-Formylcytosine antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The 5-Formylcytosine (Catalog #AAA388815) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The 5-Formylcytosine (5-fC) antibody (pAb) reacts with 5-Formylcytosine and may cross-react with other species as described in the data sheet. AAA Biotech's 5-Formylcytosine can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). Applications Validated: DB: 1:5,000 dilution. Researchers should empirically determine the suitability of the 5-Formylcytosine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "5-Formylcytosine, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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