Immunoprecipitation (IP)
(5-Methylcytosine antibody (mAb) (Clone 33D3) tested by Methyl-DNA Immunoprecipitation (MeDIP). Human DNA (500 ng) digested with Mse I was subjected to MeDIP and then analyzed by quantitative real-time PCR with primers specific for genes that are normally methylated (Xist, NYESO and SNRPN) or unmethylated (APC and p48). The MeDIP'd DNA (enriched DNA) was plotted.)
Mouse anti-Human 5-Methylcytosine Monoclonal Antibody
5-Methylcytosine (5-mC) antibody (mAb)(Clone 33D3)
Reactivity
Human
Applications
Dot Blot, ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Purity
Protein A Chromatography
Synonyms
5-Methylcytosine; Monoclonal Antibody; 5-Methylcytosine (5-mC) antibody (mAb)(Clone 33D3); anti-5-Methylcytosine antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
33D3
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in 10mM phosphate, 0.15 M NaCl and 0.01% thimerosal. Thimerosal is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-5-Methylcytosine antibody
Dot Blot (DB), ELISA (EIA), Flow Cytometry, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Methyl DNA Immunoprecipitation (MeDIP)
Application Notes
MeDIP: 1ug per IP
IHC (FFPE): 1:1000
ELISA: 1:10,000 dilution
Published Applications: MeDIP ICC/IF Flow Cytometry IHC DB
IHC (FFPE): 1:1000
ELISA: 1:10,000 dilution
Published Applications: MeDIP ICC/IF Flow Cytometry IHC DB
Conjugation
Methylated
Immunogen
Clone 33D3 recognizes the modified base 5-methylcytidine found in plant and vertebrate DNA.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Immunoprecipitation (IP)
(5-Methylcytosine antibody (mAb) (Clone 33D3) tested by Methyl-DNA Immunoprecipitation (MeDIP). Human DNA (500 ng) digested with Mse I was subjected to MeDIP and then analyzed by quantitative real-time PCR with primers specific for genes that are normally methylated (Xist, NYESO and SNRPN) or unmethylated (APC and p48). The MeDIP'd DNA (enriched DNA) was plotted.)
Immunoprecipitation (IP)
(5-Methylcytosine antibody (mAb) (Clone 33D3) tested by Methyl-DNA Immunoprecipitation (MeDIP). Human DNA (500 ng) digested with Mse I was subjected to MeDIP and then analyzed by quantitative real-time PCR with primers specific for genes that are normally methylated (Xist, NYESO and SNRPN) or unmethylated (APC and p48). The MeDIP'd DNA (enriched DNA) was plotted.)
Immunohistochemistry (IHC)
(5-Methylcytosine (5-mc) antibody (pAb) tested by Immunohistochemistry Punctate nuclear staining pattern is detected in Formalin-fixed, paraffin-embedded tissue sections from human substantia nigra (midbrain). Top Panel: 5-mC antibody at 1:1000 dilution. Bottom Panel: No primary antibody (2nd step antibody alone))
Immunohistochemistry (IHC)
(5-Methylcytosine (5-mc) antibody (pAb) tested by Immunohistochemistry Punctate nuclear staining pattern is detected in Formalin-fixed, paraffin-embedded tissue sections from human substantia nigra (midbrain). Top Panel: 5-mC antibody at 1:1000 dilution. Bottom Panel: No primary antibody (2nd step antibody alone))
Related Product Information for anti-5-Methylcytosine antibody
Short Description: Our 5-Methylcytosine (5-mC) antibody (mAb)(Clone 33D3) was raised in a Mouse host. It has been validated for use in Dot blot, ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry and Methyl DNA Immunoprecipitation; it has been shown to react with Human samples, but the sequence is not species specific so it should react with a wide range of sample types.
Background: 5-Methylcytosine (5-Methylcytidine) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. The 5-methylcytidine antibody (Clone 33D3) has been developed to discriminate between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis.
Background: 5-Methylcytosine (5-Methylcytidine) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. The 5-methylcytidine antibody (Clone 33D3) has been developed to discriminate between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis.
Product Categories/Family for anti-5-Methylcytosine antibody
NCBI and Uniprot Product Information
Protein Family
Similar Products
Product Notes
The 5-Methylcytosine (Catalog #AAA388526) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The 5-Methylcytosine (5-mC) antibody (mAb)(Clone 33D3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's 5-Methylcytosine can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), ELISA (EIA), Flow Cytometry, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Methyl DNA Immunoprecipitation (MeDIP). MeDIP: 1ug per IP IHC (FFPE): 1:1000 ELISA: 1:10,000 dilution Published Applications: MeDIP ICC/IF Flow Cytometry IHC DB. Researchers should empirically determine the suitability of the 5-Methylcytosine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "5-Methylcytosine, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
