Testing Data
(Next-Gen sequencing data generated using 5-Methylcytosine (5-mC) antibody (mAb) correlates well with CpG density. DNA was enriched from 1 ug of denatured, adaptor ligated human PC9 cell DNA using 2 ul of the 5-Methylcytosine (5-mC) antibody (mAb). MeDIP DNA was sequenced using the Illumina platform to generate 26 million sequence tags. Tags were mapped to generate a whole-genome DNA methylation profile. The image above shows that the enriched regions (purple peaks) correlate well with CpG density (green overlay).)
Mouse anti-Human 5-Methylcytosine Monoclonal Antibody
5-Methylcytosine (5-mC) antibody (mAb)(Clone A1)
Reactivity
Human
Applications
Dot Blot, Immunofluorescence, Immunoprecipitation
Purity
Protein A Chromatography
Synonyms
5-Methylcytosine; Monoclonal Antibody; 5-Methylcytosine (5-mC) antibody (mAb)(Clone A1); anti-5-Methylcytosine antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
A1
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-5-Methylcytosine antibody
Dot Blot (DB), Immunofluorescence (IF), Methyl DNA Immunoprecipitation (MeDIP)
Application Notes
Applications Validated:
MeDIP: 1-2ug per IP
MeDIP-Seq: 1-2ug each
DB: 0.5-2ug/ml dilution
In MeDIP, the DNA must be denatured when using this antibody. For 5-methylcytosine, we also offer AbFlex 5-methylcytosine Recombinant Antibody (rAb). For details
MeDIP: 1-2ug per IP
MeDIP-Seq: 1-2ug each
DB: 0.5-2ug/ml dilution
In MeDIP, the DNA must be denatured when using this antibody. For 5-methylcytosine, we also offer AbFlex 5-methylcytosine Recombinant Antibody (rAb). For details
Conjugation
Methylated
Immunogen
This 5-Methylcytosine (5-mC) antibody was raised against 5-Methyl-cytidine conjugated to KLH and recognizes 5-Methylcytosine.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Testing Data
(Next-Gen sequencing data generated using 5-Methylcytosine (5-mC) antibody (mAb) correlates well with CpG density. DNA was enriched from 1 ug of denatured, adaptor ligated human PC9 cell DNA using 2 ul of the 5-Methylcytosine (5-mC) antibody (mAb). MeDIP DNA was sequenced using the Illumina platform to generate 26 million sequence tags. Tags were mapped to generate a whole-genome DNA methylation profile. The image above shows that the enriched regions (purple peaks) correlate well with CpG density (green overlay).)
Testing Data
(Next-Gen sequencing data generated using 5-Methylcytosine (5-mC) antibody (mAb) correlates well with CpG density. DNA was enriched from 1 ug of denatured, adaptor ligated human PC9 cell DNA using 2 ul of the 5-Methylcytosine (5-mC) antibody (mAb). MeDIP DNA was sequenced using the Illumina platform to generate 26 million sequence tags. Tags were mapped to generate a whole-genome DNA methylation profile. The image above shows that the enriched regions (purple peaks) correlate well with CpG density (green overlay).)
Immunoprecipitation (IP)
(5-Methylcytosine (5-mC) antibody (mAb) tested by Methyl-DNA Immunoprecipitation (MeDIP). Methylated DNA derived from the promoter of the APC gene was amplified using 5me-dCTP and PCR. 25pg of methylated DNA was spiked into 500 ng of MseI digested human genomic DNA and subjected to the MeDIP procedure using 5?g of 5-Methylcytidine antibody or control mouse IgG. Real time quantitative PCR to amplify the methylated APC promoter DNA was performed on the immunoprecipitated DNA and results plotted as Fold Enrichment over control IgG.)
Immunoprecipitation (IP)
(5-Methylcytosine (5-mC) antibody (mAb) tested by Methyl-DNA Immunoprecipitation (MeDIP). Methylated DNA derived from the promoter of the APC gene was amplified using 5me-dCTP and PCR. 25pg of methylated DNA was spiked into 500 ng of MseI digested human genomic DNA and subjected to the MeDIP procedure using 5?g of 5-Methylcytidine antibody or control mouse IgG. Real time quantitative PCR to amplify the methylated APC promoter DNA was performed on the immunoprecipitated DNA and results plotted as Fold Enrichment over control IgG.)
Dot Blot (DB)
(5-Methylcytosine (5-mC) antibody (mAb) tested by dot blot analysis. DNA from the Methylated DNA Standard Kit were spotted (5 ng per spot) on to a positively charged nylon membrane and blotted with 5-Methylcytosine antibody (2 ug/ml dilution). Lane 1: single-stranded unmethylated DNA. Lane 2: single-stranded DNA containing 5-methylcytosine. Lane 3: single-stranded DNA containing 5-hydroxymethylcytosine. Lane 4: double-stranded unmethylated DNA. Lane 5: double-stranded DNA containing 5-methylcytosine. Lane 6: double-stranded DNA 5-hydroxymethylcytosine.)
Dot Blot (DB)
(5-Methylcytosine (5-mC) antibody (mAb) tested by dot blot analysis. DNA from the Methylated DNA Standard Kit were spotted (5 ng per spot) on to a positively charged nylon membrane and blotted with 5-Methylcytosine antibody (2 ug/ml dilution). Lane 1: single-stranded unmethylated DNA. Lane 2: single-stranded DNA containing 5-methylcytosine. Lane 3: single-stranded DNA containing 5-hydroxymethylcytosine. Lane 4: double-stranded unmethylated DNA. Lane 5: double-stranded DNA containing 5-methylcytosine. Lane 6: double-stranded DNA 5-hydroxymethylcytosine.)
Related Product Information for anti-5-Methylcytosine antibody
Short Description: Our 5-Methylcytosine (5-mC) antibody (mAb)(Clone A1) was raised in a Mouse host. It has been validated for use in Dot blot, Immunofluorescence and Methyl DNA Immunoprecipitation; it has been shown to react with Human samples, but the sequence is not species specific so it should react with a wide range of sample types.
Background: 5-Methylcytosine (5-mC) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. The 5-methylcytidine antibody (Clone A1) has been developed to discriminate between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis.
Background: 5-Methylcytosine (5-mC) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. The 5-methylcytidine antibody (Clone A1) has been developed to discriminate between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis.
Product Categories/Family for anti-5-Methylcytosine antibody
NCBI and Uniprot Product Information
Protein Family
Similar Products
Product Notes
The 5-Methylcytosine (Catalog #AAA388914) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The 5-Methylcytosine (5-mC) antibody (mAb)(Clone A1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's 5-Methylcytosine can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Immunofluorescence (IF), Methyl DNA Immunoprecipitation (MeDIP). Applications Validated: MeDIP: 1-2ug per IP MeDIP-Seq: 1-2ug each DB: 0.5-2ug/ml dilution In MeDIP, the DNA must be denatured when using this antibody. For 5-methylcytosine, we also offer AbFlex 5-methylcytosine Recombinant Antibody (rAb). For details. Researchers should empirically determine the suitability of the 5-Methylcytosine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "5-Methylcytosine, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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