Mouse anti-Human IQGAP1 Monoclonal Antibody | anti-IQGAP1 antibody

IQGAP1 (KIAA0051) (PE)

Cat. Num. AAA6244217

• Gene Names: IQGAP1; SAR1; p195; HUMORFA01
• Reactivity: Human
• Applications: Immunoprecipitation, Western Blot
• Purity: Purified by protein G affinity chromatography from ascites.
• Synonyms: IQGAP1; Monoclonal Antibody; IQGAP1 (KIAA0051) (PE); anti-IQGAP1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 0.T.72
• Specificity: Recognizes human IQGAP1 at 185kD. Does not recognize IQGAP2.
• Purity/Purification: Purified by protein G affinity chromatography from ascites.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-IQGAP1 antibody: Immunoprecipitation (IP), Western Blot (WB)
• Immunogen: A Glu-Glu tagged C-terminal fragment corresponding to aa863-1657 of human IQGAP1 purified from baculovirus infected insect cells.
• Conjugate: PE
• Preparation and Storage: Store at 4 degree C. Do Not Freeze.

Western Blot (WB)

(WB: HepG2 cell lysate probed was probed with MBS603914 (0.5ug/ml).)

Product Categories/Family for anti-IQGAP1 antibody

Antibodies; Proteins

References

1. Kuroda, S., et al., Biochem. Biophys. Res. Commun. 262: 1-6 (1999). 2. Weissbach, L., et al., Biochem. Biophys. Res. Commun. 251: 269-276 (1998). 3. Hart, M.J., et al., EMBO J. 15: 2997-3005 (1996)

NCBI and Uniprot Product Information

• NCBI GI #: 4506787
• NCBI GeneID: 8826
• NCBI Accession #: NP_003861.1
• NCBI GenBank Nucleotide #: NM_003870
• UniProt Accession #: P46940; A7MBM3
• Molecular Weight: 189,252 Da
• NCBI Official Full Name: ras GTPase-activating-like protein IQGAP1
• NCBI Official Synonym Full Names: IQ motif containing GTPase activating protein 1
• NCBI Official Symbol: IQGAP1
• NCBI Official Synonym Symbols: SAR1; p195; HUMORFA01
• NCBI Protein Information: ras GTPase-activating-like protein IQGAP1; RasGAP-like with IQ motifs
• UniProt Protein Name: Ras GTPase-activating-like protein IQGAP1
• Protein Family: Ras GTPase-activating-like protein
• UniProt Gene Name: IQGAP1
• UniProt Synonym Gene Names: KIAA0051
• UniProt Entry Name: IQGA1_HUMAN

NCBI Description

This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines. [provided by RefSeq, Jul 2008]

Uniprot Description

IQGAP1: Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth. Interacts with CDC42; the interaction is demonstrated with IQGAP1 in GTP-bound and in nucleotide-free state. Interacts with RAC1. Does not interact with RHOA. Interacts with TSG101. Interacts with PAK6. Expressed in the placenta, lung, and kidney. A lower level expression is seen in the heart, liver, skeletal muscle and pancreas.

Protein type: GAPs, Ras; GAPs; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: microtubule; neuron projection; focal adhesion; leading edge; actin filament; actin cytoskeleton; microtubule cytoskeleton; nucleoplasm; extrinsic to internal side of plasma membrane; growth cone; axon; cytoplasm; plasma membrane; midbody; cell junction; lateral plasma membrane

Molecular Function: calmodulin binding; protein serine/threonine kinase activator activity; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; Rac GTPase binding; protein complex binding; calcium ion binding; protein phosphatase binding; protein kinase binding; GTPase activator activity; GTPase inhibitor activity

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; small GTPase mediated signal transduction; positive regulation of protein kinase activity; negative regulation of dephosphorylation; negative regulation of catalytic activity; energy reserve metabolic process; platelet-derived growth factor receptor signaling pathway; signal transduction; regulation of insulin secretion; regulation of cytokine production; positive regulation of GTPase activity